Real time PCR: a rapid tool for potency estimation of live attenuated camelpox and buffalopox vaccines. Biologicals 40, 92-95.Prabhu M, Siva Sankar MS, Bhanuprakash V, Venkatesan G, Bora DP, Yogisharadhya R, Balamurugan V (2012) Real time PCR: a rapid tool for potency estimation of ...
其中,DNA样品具体制备方法为:取100 μL纯菌稀释液样品于PCR管中,以6 000 r/min的速度离心5 min,去除上清,加入30 μL无菌水充分悬浮,后续参照1.3.2进行操作。另外,各取以上8份纯菌稀释液样品100 μL,参照GB 4789.2-2016[23],经螺旋计数法测定其各自对应的含菌量。比对上述LAMP方法检测和螺旋计数结果,并...
Species radiations—wherein perplexing amounts of diversity appear to have formed extremely rapidly—have featured prominently in the history of evolutionary theory1. Various underlying mechanisms for their formation have been proposed2, including adaptation2, non-adaptive processes3,4, hybridisation5,6,...
qRT-PCR confirmed ablation of sox17 mRNA expression in 10 hpf wild-type embryos injected with sox32 Cas9 RNP (Figure 3C). Observation of sox32 G0 embryos at 24 hpf and later revealed the expected morphologic phenotypes (Figure S3A). Additional qRT-PCR of embryos collected at various times ...
Travelers flying to China from US cities including Los Angeles, New York and San Francesco from May 20 will no longer need an RT-PCR test seven days before flights, notices issued late on Tuesday by the Chinese embassy ...
Comparison of a PfHRP2-based rapid diagnostic test and PCR for malaria in a low prevalence setting in rural southern Zambia: implications for elimination. Malar J. 2015;14:25. Article Google Scholar Mabey D, Peeling RW, Ustianowski A, Perkins MD. Tropical infectious diseases: diagnostics ...
PCR. For this first RT-PCR test, the US Centres for Disease Control and Prevention N2-gene assay17as well as the Alberta Precision Labs E-gene assay16were performed. For the E-gene assay, 5 µL of extracted RNA was added to 2.5 µL of TaqMan Fast Virus One-Step RT-PCR Master ...
What's been the reaction to the Buffalo vs. New Jersey series, which many point to as being an example of on-ice transmission between teams? Wyshynski:The weekend of January 30-31 featured two games in Buffalo between the Sabres and the Devils. Following that weekend...
We targeted three fibroin genes for silencing and measured their expression levels by qRT-PCR 4 days after injection (Fig. 5b). In comparison with the noninjected groups, there were no significant changes in the expression of GFP, HcunFib-L and HcunP25 (p > 0.05), while the relative...
Development of a DNA-based screening method to detect cow milk in ewe, goat and buffalo milk and dairy products using PCR-LCR-EIA technique Milchwissenschaft, 56 (2001), pp. 67-70 View in ScopusGoogle Scholar Malferrari et al., 2002 G. Malferrari, E. Monferini, P. DeBlasio, G. Dia...