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Over this time, myoblasts differentiated into myotubes, as observed using an inverted microscope (Nikon ECLIPSE TS100), and immunofluorescence cell staining was applied to quantify the maturity of myotubes, as observed using a fluorescence microscope (Nikon ECLIPSE Ni). In addition, C2C12 cell ...
Migration of wound edges was measured at five random points on images acquired using an ECLIPSE TS100 microscope (Nikon), and the cell migration distance after 10 h was compared with the distance at 0 h. Data analysis was performed according to the previous protocol.20 Study approval and...
The method may also comprise periodically measuring the detectable signal during the isothermally amplifying, and determining at least one of an amplification threshold breach or an amplification rate corresponding to levels of the detectable signal versus amplification time. In certain embodiments, the iso...
at room temperature for 2 h in darkness. After washing with 1X PBS, sections were mounted (Antifade mounting medium, H-1000, Vector Laboratories). The stained sections were stored in the dark box at 4 °C before imaging. Images were visualized by epifluorescence (Nikon Eclipse Ni-E ...
Vandemark G, Miklas PN: Genotyping common bean for the potyvirus resistance alleles I and bc-12 with a multiplex real-time polymerase chain reaction assay. Phytopath. 2005, 95: 499-505. 10.1094/PHYTO-95-0499. Article CAS Google Scholar Miklas PN, Coyne DP, Grafton KF, Mutlu N, Reiser...
Soybean (Glycine max; SB) leaf (SL) is an abundant non-conventional edible resource that possesses value-adding bioactive compounds. We predicted the attributes of SB based on the metabolomes of an SL using targeted metabolomics. The SB was planted in tw
One swab was used to make vaginal discharge smears for Gram staining and then exanimated by an experienced clinical scientist using a microscope (Nikon eclipse 80i, Japan). When the Candida budding yeasts, hyphae, or pseudohyphae were found under the microscope, the sample was diagnosed as VVC...