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Over this time, myoblasts differentiated into myotubes, as observed using an inverted microscope (Nikon ECLIPSE TS100), and immunofluorescence cell staining was applied to quantify the maturity of myotubes, as observed using a fluorescence microscope (Nikon ECLIPSE Ni). In addition, C2C12 cell ...
Migration of wound edges was measured at five random points on images acquired using an ECLIPSE TS100 microscope (Nikon), and the cell migration distance after 10 h was compared with the distance at 0 h. Data analysis was performed according to the previous protocol.20 Study approval and...
(Biosearch Technologies), BHQ®-3 black hole quencher dye (Biosearch Technologies), BLACKBERRY® quencher (BBQ®)-650 quencher dye (Berry and Associates), ECLIPSE® quencher dye (Elitech Group), DABCYL® quencher dye, TAMRA™ fluorescent quencher dye (Applied Biosystems), and combinations...
Adult C57BL/6 mice (2–4 months old) were used in this study. Male mice were used throughout this study except for data presented in Figs.2and4, where both male and female mice were used. Mice were acclimated to the Laboratory Animal Research Unit (LARU) at the City University of Hon...
Common bean was one of the first crops that benefited from the development and utilization of molecular marker-assisted selection (MAS) for major disease resistance genes. Efficiency of MAS for breeding common bean is still hampered, however, due to the dominance, linkage phase, and loose linkage...
Soybean (Glycine max; SB) leaf (SL) is an abundant non-conventional edible resource that possesses value-adding bioactive compounds. We predicted the attributes of SB based on the metabolomes of an SL using targeted metabolomics. The SB was planted in tw
One swab was used to make vaginal discharge smears for Gram staining and then exanimated by an experienced clinical scientist using a microscope (Nikon eclipse 80i, Japan). When the Candida budding yeasts, hyphae, or pseudohyphae were found under the microscope, the sample was diagnosed as VVC...