Sigma-Aldrich 37094 Rapamycin VETRANAL 53123-88-9 10mg $595 2024-03-01 BuyRapamycin Chemical Properties,Uses,Production description Rapamycin (also known as Sirolimus; CAS: 53123-88-9) is macrocyclic lactone produced by the bacterium Streptomyces hygroscopicus isolated from soil samples from Easter ...
(Sigma, San Francisco, CA, USA) on cell behavior, normal or fibrotic HLFs (N-/F-HLFs) were incubated for different periods of time (1, 6, 12, 24, and 48 h) with rapamycin (5 ng/ml) in the absence or presence of transforming growth factor (TGF)-β1 (10 ng/ml, BD, San ...
Mol. Weight 914.17 Qty 1 50mg Qty 2 100mg Appearance White solid Application Notes 98% NMR (Conforms) Synonym Sirolimus Solubility Soluble in DMSO (up to 40 mg/ml) or in Ethanol (up to 90 mg/ml). Storage condition -20℃ Stability ...
产品名称: Rapamycin雷帕霉素 .0 订购此产品 供应商: Sigma 规格: 1mg 目录价: 5040 库存状态: 53123-88-9 CAS编号: 应用范围: 种属来源: 相关信息: R0395 SIGMA Rapamycin from Streptomyces hygroscopicus ≥95% (HPLC), powder Synonym: 23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentri...
After discarding the goat serum, the samples were incubated overnight at 4 °C with anti-LC3 antibodies (Cell Signaling Technology) and anti-RAB7 antibodies (Sigma-Aldrich). After being returned to room temperature, the sections were PBS-washed. Alexa Fluor 488-conjugated goat anti-rabbit IgG ...
Sections were deparaffinized using Histochoice (Sigma-Aldrich) and dehydrated. Dewaxed sections were microwaved for 15 minutes in 1 mM EDTA (pH 8.0), cooled, and treated with 3% H2O2 (Sigma) in 10% methanol to inhibit endogenous peroxidase activity, blocked for 15 minutes at RT in solution...
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Rapamycin, okadaic acid, retinoic acid, brain-derived neurotrophic factor (BDNF), and protease inhibitor mixture were purchased from Sigma-Aldrich Sweden AB, and culture media were from Invitrogen. Cytosolic abnormally hyperphosphorylated tau (AD p-tau) and PHF-tau isolated from an AD brain were ...
Anti-phospho-4E-BP1 (1 : 1000 for western blotting), anti- phospho-S6 ribosomal protein (1 : 1000 for western blotting), anti-mTOR (1 : 1000 for western blotting) were obtained from Cell Signaling Technology (Hertfordshire, UK) and anti-b-actin was from Sigma–Aldrich (St. Louis, MO,...
Anti-phospho-4E-BP1 (1 : 1000 for western blotting), anti- phospho-S6 ribosomal protein (1 : 1000 for western blotting), anti-mTOR (1 : 1000 for western blotting) were obtained from Cell Signaling Technology (Hertfordshire, UK) and anti-b-actin was from Sigma–Aldrich (St. Louis, MO,...