细胞营养状态通过Rag GTPases来实现对mTORC1活性的调控。其中氨基酸水平变化通过感受器-GATOR2-GATOR1来到Rag GTPase RagA/RagB操控其GTP-GDP之间的切换。而FLCN-FNIP2和亮氨酸tRNA合成酶则控制了RagC/RagD、RagD。工作时Rag GTPases是一个二聚体，必须是RagA/B（ GTP）:RagC/
细胞营养状态通过Rag GTPases来实现对mTORC1活性的调控。其中氨基酸水平变化通过感受器-GATOR2-GATOR1来到Rag GTPase RagA/RagB操控其GTP-GDP之间的切换。而FLCN-FNIP2和亮氨酸tRNA合成酶则控制了RagC/RagD、RagD。 工作时Rag GTPases是...
在营养富足的条件下,Ragulator-Rag GTPase负责招募mTORC1到生物大分子降解循环的细胞器溶酶体表面执行其功能。细胞营养状态通过Rag GTPases来实现对mTORC1活性的调控。其中氨基酸水平变化通过感受器-GATOR2-GATOR1来到Rag GTPase RagA/RagB操控其GTP-GDP之间的切换。而FLCN-FNIP2和亮氨酸tRNA合成酶则控制了RagC/RagD...
Important mechanistic questions remain unresolved, however, regarding how guanine nucleotide binding enables Rag GTPases to transition dynamically between distinct yoga-like structural poses that control activation state. Egri and Shen identified a critical interdomain hydrogen bond within RagA and RagC ...
细胞营养状态通过Rag GTPases来实现对mTORC1活性的调控。其中氨基酸水平变化通过感受器-GATOR2-GATOR1来到Rag GTPase RagA/RagB操控其GTP-GDP之间的切换。而FLCN-FNIP2和亮氨酸tRNA合成酶则控制了RagC/RagD、RagD。 工作时Rag GTPases是一个二聚体,必须是RagA/B( GTP):RagC/D (GDP)一个异源二聚体方可...
Kim E,Guan KL. RAG GTPases in nutrient‐mediated TOR signaling pathway. Cell Cycle 2009, 8: 1014‐1018.E. Kim, and K.-L. Guan, RAG GTPases in nutrient-mediated TOR signaling pathway. Cell Cycle 8 (2009) 1014-8....
The Rag GTPases appear to reside on the lysosome and link amino acid stimulation to mTOR complex 1 (mTORC1) activation. mTORC1 couples nutrient availability to cell growth. Dysregulation of mTORC1 is...doi:10.1007/978-3-319-07761-1_12Jenna L. Jewell...
Here, using several biochemical and genetic approaches, we show that eight amino acids filter through the Rag GTPase pathway. Like glutamine, asparagine signals to mTORC1 through Arf1 in the absence of the Rag GTPases. Both the Rag-dependent and Rag-independent pathways required the lysosome ...
Nutrients, such as amino acids and glucose, signal through the Rag GTPases to activate mTORC1. The GATOR1 protein complex—comprising DEPDC5, NPRL2 and NPRL3—regulates the Rag GTPases as a GTPase-activating protein (GAP) for RAGA; loss of GATOR1 desensi
Amino acid-dependent activation of the mechanistic target of rapamycin complex 1 (mTORC1) is mediated by Rag GTPases, which are recruited to the lysosome by the Ragulator complex consisting of p18, MP1, p14, HBXIP and C7orf59; however, the molecular mechanism is elusive. Here, we report ...