In this review, we focus on the mammalian RADSl structure and functionand highlight the use of model systems to enable mechanistic understanding of RADSl cellular roles. We also discuss how misregulation of the RAD)1 gene family members contributes to disease and consider new approaches to ...
RAD51 gene family structure and function Annu Rev Genet, 54 (2020), pp. 25-46 Google Scholar 4 A. Carver, X. Zhang Rad51 filament dynamics and its antagonistic modulators Semin Cell Dev Biol, 113 (2021), pp. 3-13 Google Scholar 5 I.E. Wassing, F. Esashi RAD51: beyond the break...
The RAD51 family: roles in early and late stages of HR? As noted above, RAD51 itself has the core function of strand invasion: that is, polymerizing onto a 3′ DNA end and mediating the transfer and annealing of the resulting nucleoprotein filament to a complementary homologous strand on ...
We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some...
We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some...
For the molecular function, protein binding was significantly enriched. In addition, KEGG pathway analysis showed that these genes were involved in metabolic pathways and cancer-related pathways. Figure 3 GO and KEGG analysis of DEGs. (a–d) Bubble plots showing the results of functional annotation...
The PCSS complex, comprising budding yeast Psy3, Csm2, Shu1 and Shu2 proteins, binds to recombination sites and is required for Rad51 assembly and function during meiosis. Within the hetero-tetramer, Psy3-Csm2 constitutes a core sub-complex with DNA-binding activity. In vitro, purified Psy...
Sung, P. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein.Science265, 1241–1243 (1994). ArticlePubMedCASGoogle Scholar Stasiak, A. & Egelman, E. H. Structure and function of RecA-DNA complexes.Experientia50, 192–203 (1994). ...
His postdoc work established the gene transposition model of mating-type switching of budding yeast and discovered the phenomenon of gene silencing in yeast by discovering the MAR1 gene (later named SIR2, Sirtuin) function in silencing the mating-type loci by epigenetics. He then held a ...
Strains of Saccharomyces cerevisiae carrying a deletion of the RAD51 gene are viable but are highly sensitive to ionizing radiation and are defective in meiosis (7). The S. cerevisiae Rad51 protein has been purified and shown to form nucleoprotein filaments and promote DNA strand exchange, albeit...