Rac1-GTPase慢病毒的构建及生物学活性鉴定王斌1,李娟1,张磊1,张琳2,张璐1(南方医科大学1病理生理学教研室/广东省功能蛋白质组学重点实验室,2组织学与胚胎学教研室,广东广州510515)摘要:目的构建携带绿色荧光蛋白的Rac1-GTPase显性负效突变体(Rac1N17)和组成型活性突变体(Rac1L61)的慢病毒。方法构建Rac1N17和Ra...
VEGF诱导的内皮细胞运动和毛细血管形成部分受到GTPase Rac1的调控,而CdGAP被描述为Rac1活性的调节因子。 因此,作者接下来评估CdGAP的缺失是否会影响VEGF诱导的Rac1激活。 为了实现这一点,使用融合到GST的PAK的Cdc42/Rac交互结合(...
that play a role in cancer development (Fig. 1): mitogen-activated protein kinase (MAPK) pathway, phosphoinositide 3-kinase (PI3K) pathway, Ral guanine nucleotide dissociation stimulator (Ral-GDS), and Ras-related C3 botulinum toxin substrate 1 (Rac1) GTPase. Efforts to block these effectors ...
Rac1 GTPase-deficient He La cells present reduced DNA repair,proliferation,and survival under UV or gamma irradiation. Espinha G,Osaki JH,Magalhaes YT et al. Molecular and Cellular Biochemistry . 2015Espinha G, Osaki JH, Magalhaes YT, Forti FL. Rac1 GTPase-deficient HeLa cells present re- ...
Rac1-GTPase; Hematopoietic stem/progenitor cells; Niche; Leukemia; Homing; Quiescence Abstract Interaction between hematopoietic stem/progenitor cells (HSPCs) with their niche is critical for HSPC function. The interaction also plays an important role in the multi-step process of leukemogenesis. Rac1 ...
β-catenin is a key mediator of the canonical Wnt pathway as it associates with members of the T-cell factor (TCF) family at Wnt-responsive promoters to drive the transcription of Wnt target genes. Recently, we showed that Rac1 GTPase synergizes with β-
RacGAP1 ELISA试剂盒 GTPase检测试剂盒 形状:盒装液体 贮藏条件:2-8℃低温保存 有效期:6个月 检测范围:78.125-5000pg/ml 特异性:同时检测天然或重组的,且与其他相关蛋白无交叉反应。 检测种属:人、大小鼠、牛、羊、猴、猪elisa检测试剂盒等种属。 可检测多种生物样品,包括:血清、血浆、体液如尿液、细胞培养上...
Role of Rac1-GTPase in Glucose Inhibition of Glucagon Secretionhydroperoxide lipidsGUVphotosensitizationmethylene blueNot Availabledoi:10.1016/j.bpj.2011.11.1757Elliott, Amicia D.Piston, David W.Elsevier Inc.Biophysical Journal
Physiological studies of ion channel regulation have implicated the Ser/Thr protein phosphatase 5 (PP5) as an effector of Rac1 GTPase signaling, but direct biochemical evidence for PP5 regulation by Rac1 is lacking. In this study we used immunoprecipitation, in vitro binding, cellular fractionation...
中文名称: RACGAP1(RACGTPASEACTIVATINGPROTEIN1),ID-GAP,MGCRACGAP,~60KD,GOATANTI-HUMAN:WB 中文同义词: 英文名称: RACGAP1(RACGTPASEACTIVATINGPROTEIN1),ID-GAP,MGCRACGAP,~60KD,GOATANTI-HUMAN:WB 英文同义词: RACGAP1(RACGTPASEACTIVATINGPROTEIN1),ID-GAP,MGCRACGAP,~60KD,GOATANTI-HUMAN:WB CAS号...