6 QuikChange Site-Directed Mutagenesis Kit PROTOCOL Mutant Strand Synthesis Reaction (Thermal Cycling) Notes Ensure that the plasmid DNA template is isolated from a dam+ E. coli strain. The majority of the commonly used E. coli strains are dam+. Plasmid DNA isolated from dam– strains (e.g...
QuikChange Site-Directed Mutagenesis Kit 7 PROTOCOL Mutant Strand Synthesis Reaction (Thermal Cycling) Notes Ensure that the plasmid DNA template is isolated from a dam+ E. coli strain. The majority of the commonly used E. coli strains are dam+. Plasmid DNA isolated from dam– strains (e.g...
Storage Conditions: All components of the QuikChangeLightning Site-Directed Mutagenesis Kit should be stored at -20°C, excluding the ultracompetent cells, which should be stored immediay at -80°C. Test ConditionsFollowing the protocol outlined in the instruction manual, the pWhitescript™ 4.5-...
上海玉博生物科技有限公司 主要致力于“点突变试剂盒,QuikChange Lightning Site-Directed Mutagenesis Kit Stratagene 210518”的生产销售。多年的“点突变试剂盒,QuikChange Lightning Site-Directed Mutagenesis Kit Stratagene 210518”生产与销售的经验,与各行业
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QuikChange Lightning Multi Site-Directed Mutagenesis Kit (academic) Instruction manual for the academic version of QuikChange Lightning Multi Site-Directed Mutagenesis Kit with introduction, guidelines and protocol requirements. 用户手册 English 2023 年 9 月 8 日 ...
The new generation of the Stratagene QuikChange Lightning Site-Directed Mutagenesis Kit advances our mutagenesis technology to the next level. Introduce point mutations, insertions or deletions signif i cantly faster than our original kits. Our specially engineered enzymes have been designed to shorten ...
6 QuikChange II Site-Directed Mutagenesis Kit Additional Primer Considerations ? The mutagenesis protocol uses 125 ng of each oligonucleotide primer. To convert nanograms to picomoles of oligo, use the following equation: X pmoles of oligo = ng of oligo × 1000 330 × # of bases in oligo ...
XLII试剂Kit突变试剂盒kit定点突变Site 系统标签: quikchangemutagenesis定点突变kit试剂directed QuikChangeIIXLSite-DirectedMutagenesisKitInstructionManualCatalog#200521(10reactions)and#200522(30reactions)RevisionE.01ResearchUseOnly.NotforUseinDiagnosticProcedures.200521-12LIMITEDPRODUCTWARRANTYThiswarrantylimitsourliabilit...
QuikChange Multi Site-Directed Mutagenesis Procedu Mutagenesis Mutagenesis PCR I (Multiple Site Directed Mutagenesis)Mixture •25µl total reaction volume :1. 2.5 µl of 10X Taq lligase buffer (need the NAD for Taq ligase)2.0.5 µl 100mM ATP 3.X µl (50-100 ng) of dsDNA ...