一般分子生物学实验室，大多能够完成PCR/电泳/胶回收/转化/细菌培养，所以对常规试剂与仪器将不再列表。对于有钱的土豪，Master P自然要列出Bigger Than Bigger的试剂,全是大牌进口：如Agilent 公司的Quickchange Site-Directed Mutagenesis Kit,New England Biolabs公司的Q5 Site-Directed Mutagenesis Kit,ThermoFisher公司...
PCR, qPCR and qRT-PCR|Takara Bio Site-Directed Mutagenesis Tips and Tricks ByTakara Bio There are a few different ways of approaching site-directed mutagenesis. Here, I’ll give you a quick introduction to inverse PCR and why it’s useful, as well as going through a full protocol for SDM...
quickchangestratagenemutagenesis定点突变kitdirected 1MarieAngePREPARATIONOFTHESINGLEANDDOUBLEMUTANTSOFFecA2CysteineMutant-QuickChangeSite-DirectedMutagenesisKit(stratagene)1.MaterialsProvided2.StorageConditionsXL1-Bluesupercompetentcells:–80°C upstairsAllOtherComponents:–20°Cfreezer upstairs3.MethodOverview34.Primer...
QuikChange®Site-DirectedMutagenesisKitINSTRUCTIONMANUALCatalog#200518(30reactions)and#200519(10reactions)RevisionAForInVitroUseOnly200518-12L..
P. (2012) Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis. Methods Mol. Biol. 944, 163-174Bok J. W., Keller N. P. ( 2012. ). Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis . Humana Press;...
each of which contains a single mutation (base change or amino acid substitution that involves one or more base changes). The latter approach is acceptable when the desired mutations are far apart. It can also be applied to closely located mutations, but the mutagenesis must be performed sequent...
Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim m
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Such factor could also limit the exchangeability of the constructs; however, our approach is affordable and straightforward allowing for use of certain elements inapt for mutagenesis. In addition, we demonstrate the programming of polycistronic mRNAs, a valuable tool for managing bista- bility/...