As used herein, the term “sample” or “test sample” may comprise biological samples, isolated nucleic acids, or isolated microorganisms. In some embodiments, a sample is obtained from a biological source (i.e., a “biological sample”), such as tissue, bodily fluid, or microorganisms colle...
Forward: 5′-GCTCAGCTCCGTTTCGGTTTCACTTCCGGT-3′ (SEQ ID NO: 13) Reverse: 5′-AGCCCCGCACTTCCACTTTCGGTTTCACTTCCGGT-3′ Primers Flanking the CCG Repeat Region of FMR2 Gene:Methods for designing suitable primers that hybridize to the 5′-UTR of the FMR2 gene as exemplified ...
Construct and test bait and wt prey plasmid 2. Construct an allele library of your prey plasmid (see SureFrame™ Allele Library Construction Kit manual) 3. Transform yeast cells with bait plasmid and library 4. Select for reporter activity by growth on selective plates 5. Confirm interaction ...