Simplexa Direct kits (Focus Diagnostics, Cypress, Calif.) contain all reagents for on-board extraction and real-time PCR. For each reaction on the Direct Amplification Disc, unprocessed CSF sample was loaded directly into a sample well of one wedge of the Disc without any sample preparation ste...
Tassone et al., “A Rapid Polymerase Chain Reaction-Based Screening Method for Identification of All Expanded Alleles of the Fragile X (FMR1) Gene in Newborn and High-Risk Populations,” Journal of Molecular Diagnostics, vol. 10, No. 1, pp. 43-40, Jan. 1, 2008. European Search Report...