the kit contains a quantified Positive Control (PosC, 20,000 copies per µL) that can be used for construction of a dilution series for HIV RNA quantification.Norgen’s HIV Quantitative RT-PCR Kit was developed and validated to be used with the following PCR instruments:Qiagen Rotor-Gene Q...
Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells - Santos - 2006 () Citation Context ...NA was extracted with a genomic DNA extraction kit (Qiagen, Valencia, CA). Quantification of mtDNA damage was done by a quantitative PCR (qPCR) that...
For the first titration series, the REPLI-g Whole Genome Amplification kit (Qiagen) was used to produce unmethylated DNA following the manufacturer's protocol. Part of the whole genome amplified DNA was incompletely methylated in vitro using M.SssI (New England BioLabs) such that ∼20% of all...
000 copies9. A PCR error at the initial step of the cluster generation process (or at the second step, in case of inefficient amplification) can produce a dominant erroneous variant that will be read from the cluster13. These errors can limit the accuracy of ultra-...
To investigate the effect of different PCR master mixes on the performance of miR-specific qPCR with DNA primers we compared the amplification of synthetic templates with the QuantiFast SYBR Green PCR master mix (Qiagen, Germany) and the Brilliant III Ultra-Fast QPCR Master Mix (Agilent, USA)...
PCR fragments were run on a 2% agarose gel, excised and eluted using the QIAquick PCR purification kit (Qiagen, USA). PCR fragments were quantified using the PicoGreen kit (Molecular Probes, The Netherlands) on a TD-360 fluorometer (Turner Design) and the molar concentration of each PCR ...
NanoDrop 1000 Quantitative - Real - Time -RT- PCR :纳米微滴1000定量实时逆转录-聚合酶链反应真实 热度: RTI Press Quantitative Real-Time Polymerase Chain Reaction (qPCR) of Filamentous Fungi in Carpet Jonathan Black September 2009 Methods RepoRt ...
Kit(Qiagen,Hilden,Germany).Prior toDNAextraction、a microdissection technique wasusedincertaincaseswhen tumortissuesandnon—tumortissuesweremixedinone sample toenrichtumorcellsi20}.Five10一Bmthicksectionswerecut and placed intoa1.5mL Eppendorff tube.Thesectionswere deparaffinizedtwicewithxyleneandalcoh01. ...
(Fig.7, see Supplementary Data16for the table format). HNF4A, whose mRNAs were found to be upregulated in the blood of 51 PD patients vs. 45 controls using quantitative PCR assays, was significantly upregulated in PD (p < 0.01). Meanwhile, APP, whose mRNAs have been shown as ...
An equal volume (6 μL) of each PCR product was pooled together. The concentration of the pooled PCR products was measured using the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32854). Pooled PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, #28106) with ...