使用类绿色 (Qiagen) 进行定量实时 PCR (qPCR)。在表 1 中列出了所选的基因的引物对。 翻译结果4复制译文编辑译文朗读译文返回顶部 在数量上实现实时pcr(qpcr)是执行使用sybr绿色(qiagen)。 手油泵对基因的选择列在表1中。 翻译结果5复制译文编辑译文朗读译文返回顶部 定量真正的时间PCR (qPCR)使用SYBR绿色(Qiage...
Fungal DNA was extracted using the DNeasy Plant Mini Kit (Qiagen, Dusseldorf, Germany) according to the manufacturer’s instructions, with adaptations of the initial steps. The first step was to determine whether additional homogenization processes could increase the yield of DNA extracted from the ...
Plasmids containing these BV sequences were constructed using a Zero Blunt TOPO PCR Cloning Kit (Invitrogen, Waltham, MA, USA). Plasmids were digested with EcoRI. DNA fragments were electrophoresed on agarose gels and purified using a QIAquick Gel Extraction Kit (QIAGEN). The purified DNAs ...
Quantitative RT-PCR ReadyMix™ (One step RT-qPCR for probe-based methods, MMLV & hot-start Taq); The Quantitative RT-PCR ReadyMix kit includes Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), for efficient first strand cDNA;
For the first titration series, the REPLI-g Whole Genome Amplification kit (Qiagen) was used to produce unmethylated DNA following the manufacturer's protocol. Part of the whole genome amplified DNA was incompletely methylated in vitro using M.SssI (New England BioLabs) such that ∼20% of all...
Recommended:SYBR Green kits are available from many companies. I recommendQiagen’s QuantiTect SYBR Green PCR Kit (Cat. No. 204143 for 500 rxns, Cat. No. 204145 for 2500 rxns) You will also need an internal control as a point of comparison for your data. Choose a housekeeping gene that...
PCR amplification. By introducing a sampling bottleneck after the first PCR reaction, we were able to discriminate errors introduced during that PCR procedure from those that are introduced in subsequent amplification and sequencing steps. Using this approach, we have observed specific PCR error ...
Genomic DNAs were extracted using the QIAquick PCR Purification Kit (Qiagen) from leaf tissues. Full sequencing was processed in the National Instrumentation Center for Environmental Management (NICEM) in Seoul National University, Seoul, Korea. The sequencing yielded 26X and 31X fold coverage, as...
\\{PCR\\}\\{DNA\\}extraction\\{QIAamp\\}ProteinaseKBromelainPapainWe compared four proteases in the QIAamp DNA Investigator Kit (Qiagen) to extract DNA for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate alternate proteases for improved DNA recovery as ...
The PCR efficiency (calculated from the standard curve) and correlation coefficient (R2) are shown in Table 1. The primer efficiency ranges between 93% and 105% with R2 of 0.995–1.000. These data indicate that amplification efficiencies of the primers met the standard requirement of ...