使用类绿色 (Qiagen) 进行定量实时 PCR (qPCR)。在表 1 中列出了所选的基因的引物对。 翻译结果4复制译文编辑译文朗读译文返回顶部 在数量上实现实时pcr(qpcr)是执行使用sybr绿色(qiagen)。 手油泵对基因的选择列在表1中。 翻译结果5复制译文编辑译文朗读译文返回顶部 定量真正的时间PCR (qPCR)使用SYBR绿色(Qiage...
The LightCycler PCR and detection system (Roche Diagnostics) was used for amplification and quantification of the OT-1 transgene from each tissue sample. The LightCycler FastStart DNA Master Hybridization Probes kit was used as described by the manufacturer. Each PCR mixture contained 250 ng of genom...
This kit will work on many tissues and cell types. For aorta, I useQiagen’s RNeasy Fibrous Tissue Mini Kit (Cat. No. 74704) 2.) In order to preserve RNA samples, which are very vulnerable to degradation at room temperature, I recommend using a first-strand DNA synthesis on your RNA ...
The PCR amplicons of the different microorganisms were purified using the QIAquick® PCR Purification Kit (QIAGEN GmbH, Hilden, Germany) and quantified by comparison to a DNA ladder after gel electrophoresis. Subsequently, the number of copies per μl PCR product of each amplicon was calculated....
PCR amplification. By introducing a sampling bottleneck after the first PCR reaction, we were able to discriminate errors introduced during that PCR procedure from those that are introduced in subsequent amplification and sequencing steps. Using this approach, we have observed specific PCR error ...
(Dynabeads Protein A, Thermo Fischer). Eluted DNA was purified (MinElute PCR Purification Kit; Qiagen Inc., Valencia, California, USA), then quantified with a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA). ChIP-seq libraries were prepared from two biological replicates ...
For the first titration series, the REPLI-g Whole Genome Amplification kit (Qiagen) was used to produce unmethylated DNA following the manufacturer's protocol. Part of the whole genome amplified DNA was incompletely methylated in vitro using M.SssI (New England BioLabs) such that ∼20% of all...
\\{PCR\\}\\{DNA\\}extraction\\{QIAamp\\}ProteinaseKBromelainPapainWe compared four proteases in the QIAamp DNA Investigator Kit (Qiagen) to extract DNA for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate alternate proteases for improved DNA recovery as ...
Primer specificity of the 15 candidate genes was confirmed by PCR using Fast-Run Hotstart PCR kit (Protech) and electrophoresis. Quantitative PCR. Quantitative PCR was conducted in 48-well reaction plates using the Eco Real-Time PCR System (Illumina, San Diego, CA, USA). Reactions were ...
Total RNA was extracted from the collected samples using the Trizol Reagent (Invitrogen, Shanghai, China) and the RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. The RNA purity was measured with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Rock...