61P APPLICATION OF REAL TIME QUANTITATIVE PCR FOR THE ANALYSIS OF CANCER BIOMARKERSActa Clin Belg. 2014 Nov 5:2295333714Y0000000034. [Epub ahead of print]doi:10.1016/S0169-5002(09)70184-9RassiH.Elsevier Ireland LtdLung Cancer
The PCR product is detected with a TaqMan probe. Although the method requires a specific RT primer for each miRNA, this method can be performed as multiplex so that one RT reaction can be used as template for several qPCR reactions [11]. Unfortunately, stem-loop RT-PCR does not allow the...
(iv) Bisulfite pyrosequencing (Pyroseq) applies sequencing by synthesis39 to single PCR amplicons obtained from bisulfite-converted DNA40,41. Second, relative DNA methylation assays measure DNA methylation by comparing samples to a suitable reference. This approach is mainly used for detecting ...
In the present study, we report the development and the performance of a single-tube, one-step, multiplex TaqMan RT-PCR assay (mERPR+HER2) to detect ER, PR, and HER2 mRNA levels in breast cancer FFPE specimens using two Real-Time PCR instrument platforms. In addition, we also report the...
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene have been recognized as genetic risk factors for Parkinson’s disease (PD). However, compared to cancer, fewer genetic mutations contribute to the cause of PD, propelling the search for protein b
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene have been recognized as genetic risk factors for Parkinson’s disease (PD). However, compared to cancer, fewer genetic mutations contribute to the cause of PD, propelling the search for protein b
Germ-free status was monitored by PCR (using the 16S primer pair; Supplementary Data 7) and culture-based methods. Mice were provided with standard, autoclaved chow (1318P FORTI, Altromin) ad libitum. Animals of mixed gender at an age between 8 and 17 weeks were used for all ...
The specificity of the primers designed and their potential RNA Extraction, cDNA Synthesis, and Quantitative PCR RNA was purified from FFPE tissues using the RecoverAll Total Nucleic Acid isolation Kit (Life Technologies), followed by TURBO DNase (Life Technologies) treatment to remove genomic DNA....
4.A diagnosis kit for detecting biomarkers associated with ESA-induced tumor progression, comprising: at least one container means for accepting a tumor sample; at least one reagent for carrying out PCR amplification; reagents for carrying out RNA extraction, cDNA synthesis, and pre-amplification; ...
Methods are provided for diagnosing in a subject a condition, such as a carcinoma, sarcoma or leukemia, associated with hypermethylation of genes by isolating the genes from tissue containing as few as 50 to 1000 tumor cells. Using quantitative multiplex methylation specific PCR (QM-MSP), multiple...