The sensitivity and reproducibility of Bio-COBRA make it a valuable tool for the analysis of DNA methylation in clinical samples, which could aid in the development of diagnostic and prognostic parameters with respect to disease detection and management.Brena RMAuer HKornacker KHackanson BRaval A...
Accordingly, this novel method is anticipated to be an efficient and economical alternative tool for rapid quantification of methylation patterns in screening large numbers of clinical samples across multiple genes. Laboratory Investigation (2010) 90, 282–290; doi:10.1038/labinvest.2009.132; published ...
After centrifugation at 600 rpm for 10 min, 0.7 mL of the upper phase were transferred to a vial for analysis of fatty acid methyl esters (FAMEs) by gas-chromatography. The direct transmethylation for GC analysis can be performed for Arabidopsis seeds, which contain oil approximately 35%30. ...
Mitochondrial DNA quantification as a tool for embryo viability assessment: retrospective analysis of data from single euploid blastocyst transfers. Hum Reprod. 2017;32(6):1282–92. https://doi.org/10.1093/humrep/dex070. Article CAS PubMed Google Scholar Diez-Juan A, et al. Mitochondrial DNA...
of mitoepigenetics [29, 30], its failure as a health tool marker [8] or its description as having an unusual CpG pattern.[14] Also contributing to this confusion might have been the use of quantification techniques such as ELISA and MeDIP that may struggle with low-level methylation. ...
We believe SPEAQeasy will be a useful bridging tool for those accustomed to the Bioconductor world of RNA-seq analysis.Availability of data and materials SPEAQeasy makes use of test samples, which were originally obtained SRA, Rail-RNA [67], or generated using polyester [64] (Implementation: ...
van Breukelen B, van den Toorn HWP, Drugan MM, Heck AJR (2009) StatQuant: a post-quantification analysis toolbox for improving quantitative mass spectrometry. Bioinformatics 25:1472–1473 Article Google Scholar Cox J, Neuhauser N, Michalski A, Scheltema RA, Olsen JV, Mann M (2011) Andromeda...
(ELISA) and sensitivity of polymerase chain reaction (PCR), PLA transforms the detection of protein into the detection of DNA nucleic acid sequence. Therefore, as an ultrasensitive protein assay, PLA has great potential for quantification, localization of protein, and clinical diagnostics. In this ...
Strand-specific qPCR primers OCS (59-GGACTTTACATGGGCCTTCAC-39) and OVS (59-GAAGGCTGAACTTCGACAGC-39) were designed against conserved regions of old-word begomovirus CP ORFs using the Primer Blast tool (http://www. ncbi.nlm.nih.gov/tools/primer-blast/). For a nucleotide alignment of ...
VDJ-seq is a DNA-based, high-throughput sequencing method that uses biotinylated J gene primers and UMIs to quantify immunoglobulin diversity in an unbiased manner on a per-cell basis. For high-throughput sequencing and quantification of immunoglobulin r