比对前(Pre-alignment): 处理“原始数据(raw data)” 比对后(Post-alignment): “数据过滤(data filtering)” 2. 序列质量检测及可视化软件 FastQC: 只能用于检查测序质量,不能用于质控处理。 PRINSEQ: 除了可以检测测序质量,还能用于质控处理。 2.1 FastQC的安装和使用 FastQC简介[1] FastQC是一个Java程序。既有...
Quality controlBig dataStream processingApache SparkNext generation sequencing (NGS)TOOLBackground: Quality control of DNA sequences is an important data preprocessing step in many genomic analyses. However, all existing parallel tools for this purpose are based on a batch processing model, needing to...
control samples across a range of genome sizes to illustrate that QC inferences made using draft assemblies are broadly equivalent to those made using a well-established reference, and describe QC tools routinely used in our production facility to assess the quality of NGS data from non-model ...
While guidelines for quality management in NGS data have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here,...
NGS 文库制备过程中底物耗竭后过度扩增可导致所谓“雏菊链”的形成,荧光方法无法正确定量。TapeStation 系统与专用的高灵敏度检测试剂盒为简单鉴定所需的 NGS 文库和不需要的雏菊链提供了出色的分辨率。 体验可靠的雏菊链鉴定 资料库 重要资料 Quality Control of NGS Libraries with Daisy Chain Molecule...
relatively high base error calling and thus incorrect mapping. Additionally, regions adjacent to clipped bases are usually noisy in SMS reads, especially for CSEs. CRAQ can identify the error breakpoint within the noisy region (if the NGS data show a mapping gap) with the option ‘--error_...
使用trimmomatic进行数据质控 Data QC with trimmomatic 可以使用trimmomatic -h命令查看使用帮助,得到如下反馈。 代码语言:txt 复制 PE [-version] [-threads <threads>] [-phred33|-phred64] [-trimlog <trimLogFile>] [-summary <statsSummaryFile>] [-quiet] [-validatePairs] [-basein | ] [-baseout...
Next-generation sequencing (NSG) is an important method for gathering large amounts of sequencing data for different types of applications regarding the diagnosis and response to treatment of different diseases. An important step in the NGS process is the quality control of sequencing libraries, ...
However, many of them are accessible as a command line interface that requires the user to enter each command manually. Here, we report EasyQC, a tool for NGS data quality control (QC) with a graphical user interface providing options to carry out trimming of NGS reads based on quality, ...
This Web Collection presents the results of the Sequencing Quality Control 2 (SEQC2) project that sought to evaluate quality-control metrics and human, bacterial and metagenomic reference materials and datasets for next-generation sequencing (NGS) in both regulatory settings and precision medicine. Dat...