97°C下10分钟;94°C下60分钟扩增效率:≥105倍5'–>3'外切酶活性:有Extra A辅助剂: 有3'–>5'外切酶活性:无污染核酸:无污染RNA酶:无污染蛋白酶:无自吸泵活性:无PrincipleTaq PCR Core Kit包含所有简单可靠的PCR所需的所有试剂,Taq DNA Polymerase、QIAGEN PCR Buffer、CoralLoad PCR Buffer、Q-Solution、...
阿拉丁的UltraBio™ SYBR Green qPCR Mix (2X, 防污染型),即UltraBio™ SYBR Green qPCR Mix (2X, UDG),是一种用于实时荧光定量PCR,即qPCR (Quantitative PCR)或real-time PCR的新型高品质防污染(Carryover prevention)预混液,主要用于cDNA和基因组DNA等的特异性超高灵敏度定量检测。本产品含有优化比例的...
SYBR Green I Excitation 494 nm SYBR Green I 的检测原理 Emission 521 nm 熔解曲线分析引物二聚体 Primer dimers Product of Interest UNG预防污染 52°C 2:00 UNG treatment 95°C 10:00 enzyme activation 60°C 1:00 annealing / extension 95°C 0:15 denat. UNG酶的作用原理是降解含有dU的双链或...
Human SPTBN2 qPCR Primer Pair,即人SPTBN2 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),...
Principle RT2SYBR Green qPCR Mastermixes包含real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸和SYBR Green染料。有些预混液包含荧光素或ROX染料,用于优化光学仪器。化学修饰并严格控制的HotStart酶可避免扩增引物二聚体和其他非特异性产物,提供准确的SYBR Green结果。
一般进行 qPCR 定量检测结果统计时循环数在 18-25 之间,则 数据分析结果比较可信,因此建议 miRNA qPCR 时 的循环数设置在 35x 即可.(3)qPCR 方法选择.在 miRNA 进行 qPCR 检测的众多方法之中,何种是最 好的尚无定论,从检测特异性及实验花费来说有相 同的趋势 :特异探针法≥通用探针法≥ Sybr green 染料...
and melt curve:cUsing 103copies of template 014, which contains a mismatch in each ADR, optimal PDR degeneracy was determined empirically.dThe 2830F-99% PDR variant with either the 2896R-95% (blue) or 2896R-99% (yellow) variant in a SYBR green qPCR with the 014 template showed a simil...
Working principle:The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity MPPC unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with m...
This chapter summarized the experimental principle, material preparation, operation process, and matters needing attention of SYBR Green-qPCR assay in sxtA gene detection of saxitoxin-producing cyanobacteria.Zhou, ZhongkunSchool of Pharmacy, Lanzhou UniversityMa, Yunhao...
This product uses the dye qPCR method (SYBR Green I) for accurate quantification of libraries in the Illumina sequencing process. The kit provides DNA reference standards, SYBR qPCR Master ... Compare this product infection test kitAccuPower® hepatitis C HCV clinical Contact infection test ...