优势二:累计完成上万例 RNA甲基化测序样本,全面覆盖医口、农口等各类样本。 优势三:全面检测mRNA和各类非编码RNA(circRNA,lncRNA,Pri-miRNA等)。 优势四:提供m6A一站式服务:m6A RNA修饰整体水平检测、m6A测序、MeRIP-qPCR验证、RIP和RNA pull-down、Ribo-seq、CHIP-seq等技术服务。 优势五:率先研发超微量MeRIP测...
[1] Liu, L. , Wang, J. , Sun, G. , Wu, Q. , & Pan, Q. . (2019). M6a mrna methylation regulates ctnnb1 to promote the proliferation of hepatoblastoma. Molecular Cancer, 18(1). [2] Tong, J. , Wang, X. , Liu, Y. , Ren...
Let’s suppose you use qPCR to determine the expression of oncogenes or tumor suppressor genes in a tumor biopsy sample. These clinical samples are difficult to collect and generally only contain a small amount of usable mRNA. In a singleplex relative quantificati...
形成 BCR-ABL 融合基因,该基因可转录出一个 8.5kb 的异常 mRNA,最终翻译成 210kD 大小的蛋白质——P210,P210 具有较强的酪氨酸蛋白激酶活性,可通过多种信号转导途径来活化原癌基因和某些细胞因子,导致细胞的恶性转化。
For example, when examining the effect of drug treatment on the gene expression level of a mouse mRNA target, multiple mice are needed as samples to estimate the variation of that target in the population. In this example, a biological group might con...
7、cations of RT-PCR Cloning genes expressed forms (not genomic version) Monitor a genes expression level in any tissue Monitor expression changes following treatments sequencing a whole mRNA profile EST (Expressed Sequence Tags) database Microarray (DNA chip) Diagnose and easily differentiate between...
pastoris integrated levansucrase gene in the range of 1 to 3 copies, with increase of copy numbers, the mRNA transcription level and corresponding protein expression level of multi-copy strain were significantly increased compared to the single-copy strain (P<0.0...
(day 0, 4, and 8). Primer sets for the +1kb region downstream of the transcription start sites of the 84 genes and 12 control regions were preloaded on the ChIP PCR Array. Cluster analysis (http://www.sabiosciences.com/chippcrarray_data_analysis.php) of histone marks and mRNA level ...
(day 0, 4, and 8). Primer sets for the +1kb region downstream of the transcription start sites of the 84 genes and 12 control regions were preloaded on the ChIP PCR Array. Cluster analysis (http://www.sabiosciences.com/chippcrarray_data_analysis.php) of histone marks and mRNA level ...
[4] Yang, X. , Shao, F. , D Guo, Wang, W. , & Lu, Z. . (2021). Wnt/β-catenin-suppressed fto expression increases m6a of c-myc mrna to promote tumor cell glycolysis and tumorigenesis. Cell Death & Disease, 12(5). [5] Wang, W. , Shao, F. , Yang, X. , Wang, J. ...