HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a high expressed gene, GNB2L1.HCT116 cells...
Application-specific quality control ISO 9001-compliance, with complete traceability and process controls Request a quote OEM PCR and qPCR plastics guide Play Mute Current Time 0:00 / Duration 0:00 Loaded: 0% ShareFullscreen This is a modal window. The ...
Biosearch has formulated fluorophores that emit signals from the UV to the IR, and available for 5’, 3’-, and internal-labeling of oligos. This provides maximum versatility to multiplex any probe format upon any real-time thermal cycler. Please view ourfull offering of probes with different ...
TaqPath DuraPlex 1-Step RT-qPCR Master Mix provides a full workday (over eight hours) of stability of completely assembled reactions (master mix, sample, and assay), helping enable the use of liquid handling and other automation equipment. This fast-cycling master mix can be used effectively ...
TaqPath DuraPlex 1-Step RT-qPCR Master Mix provides a full workday (over eight hours) of stability of completely assembled reactions (master mix, sample, and assay), helping enable the use of liquid handling and other automation equipment. This fast-cycling master mix can be used effectively ...
The editor, RDML-Ninja, has been designed with different user types in mind. In the laboratory, RDML-Ninja should allow researchers to enter information into RDML elements not supported by the software of their qPCR instrument. Furthermore, RDML-Ninja can form a bridge between software sup- ...
The choice of extraction method for nucleic acids has an impact on the overall quality and quantity of the extracted material as well as the target. It is therefore critical during method development to select an optimized method for different sample types. The extraction method used for a ...
Full size image To achieve cleanroom-free, low-cost fabrication, we have developed a series of fabrication methodologies based on wet etching to form porous Silicon, electroplating, thermal bonding, and laser-cutting. The fabrication of multilayered, composite TriSilix chips (dimension of each chip...
If the Vn/n+1 is super- ior to the cut-off value 0.15, the addition of the (n + 1)th gene has a significant effect on normalization quality and should preferably be included for calculation of a reliable normalization factor. In-silico analysis of RNA-seq data Three RNA-seq datasets ...
Full size image The resolution of an assay reflects its ability to discriminate between consecutive steps of the dilution series and consequently depends on the precision and accuracy of an assay. We assessed the resolution of each assay by using a pairwise T-test to evaluate whether the average...