Y.; MORZHAKOVA, A. A.; GULEVICH, A. Y.; DEBABOV, V. G. Manipulating pyruvate to acetyl-CoA conversion in Escherichia coli for anaerobic succinate biosynthesis from glucose with the yield close to the stoichiometric maximum. Journal of Biotechnology, v. 214, p. 33-42, 2015....
The pyruvate dehydrogenase complex (PDHC) catalyzes the irreversible oxidation of pyruvate to acetyl CoA according to the reaction: $$Pyruvate + NA{{D}^{+}}+CoASHo Acetyl CoA + NADH + C{{O}_{2}}$$ The acetyl CoA formed is then either oxidized in the tricarboxylic acid cycle or used ...
Why is pyruvate converted into acetyl CoA prior to entering the Krebs Cycle? What does this conversion do to the pyruvate molecules? The Krebs Cycle: The Krebs Cycle (also known as the citric acid cycle) refers to the serious of process...
to form oxaloacetate. this latter reaction "fills up" the amount of oxaloacetate in the citric acid cycle, and is therefore an anaplerotic reaction , increasing the cycle's capacity to metabolize acetyl-coa when the tissue's energy needs (e.g....
The pyruvate dehydrogenase complex (PDC) catalyzes the irreversible decarboxylation of pyruvate into acetyl-CoA. PDC deficiency can be caused by alterations in any of the genes encoding its several subunits. The resulting phenotype, though very heterogeneous, mainly affects the central nervous system. ...
2.1 Reaction and physiological context PFOR (EC 1.2.7.1) catalyzes the TPP-dependent oxidative decarboxylation of pyruvate with production of acetyl-CoA and reducing equivalents typically harvested by electron transfer to external ferredoxins (Fd) (see Eq. (1)). (1)pyruvate+CoASH+2Fdox⇄CO2+ace...
Pyruvate carboxylase (PC) was purified to homogeneity from an overexpressing strain of the purple photosynthetic bacterium Rhodobacter capsulatus using a rapid dye-ligand affinity chromatography procedure, in which dye-bound enzyme was specifically eluted with a low concentration of acetyl-CoA, an alloste...
Pyruvate oxidation is a biochemical reaction that involves the oxidation of pyruvate to create acetyl CoA. Oxidation is defined as either the loss of electrons or the gaining of oxygen. In this case, pyruvate loses electrons in the process of creating acetyl CoA. Pyruvate is the end product of...
Endogenous pyruvate decarboxylase activity in yeast converts pyruvate to acetaldehyde, which is then converted to ethanol or to acetyl-CoA via acetate (see FIG. 1). Yeasts may have one or more genes encoding pyruvate decarboylase. For example, there is one gene encoding pyruvate decarboxylase in...
Pyruvate:ferredoxin oxidoreductase (PFOR) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA and CO(2). The catalytic proficiency of this enzyme for the reverse reaction, pyruvate synthase, is poorly understood. Conversion of acetyl-CoA to pyruvate links the Wood-Ljungdahl pathway of ...