In addition, polypeptides encoded by said polynucleotides, antibodies to said polypeptides and methods for their production are provided. Further described are methods for increasing the acetyl CoA synthesis as well as methods for the production of fatty acids, carotenoids, isoprenoids, vitamins, ...
Acetyl-CoA unter aeroben Bedingungen. Die Pyruvat-Formiat-Lyase katalysiert die Umsetzung von Pyruvat zu Acetyl-CoA und Formiat unter anaeroben Bedingungen und wird bereits bei geringen Sauerstoff-Konzentrationen vollst�ndig inaktiviert. Die Pyruvat-Oxidase katalysiert die Oxidation von ...
1.Dieoptimalen BedingungenzurMessungderPyruvatdehydrogenaseüber[l-14C]PyruvatinHerz,LeberundGehirn werden beschrieben.2.InWistar-Ratten wurdebeifortschreitendemThiaminmangeldieAktivitätderPyruvatdehydro-genaseund derAbfallvoneingebautem[14C]Thia-mininHerz, LeberundGehirn gemessen. WährenddieEnzym...
In contrast to PC from mammals and another fungus, the enzyme from Phycomyces is active in the absence of acetyl-CoA. The apparent K m values for the substrates pyruvate and ATP wee determined and compared to those for PC from other fungi....
The dihydrolipoate acetyltransferase (acetyl-CoA:dihydrolipoate S-acetyltransferase, EC 2.3.1.12), the core of the enzyme complex, has been isolated by a reductive dissociation of the complex in an alkaline medium and was then purified by sucrose density gradient centrifugation. As shown by the ...
Here, we investigated whether the transfer of acetyl-CoA from the mitochondria to the cytoplasm can occur using a pyruvate decarboxylase negative, non-fermentative yeast strain. We found that mitochondrial Ach1 can convert acetyl-CoA in this compartment into acetate, which crosses the mitochondrial ...
Since the cell-free preparation had a high phosphotransacetylase activity, acetyl phosphate could be substituted for acetyl-CoA. Pyruvate could be synthetized not only from 14 CO 2 but also from H 14 COOH, however, in the latter case the specific activity of the resulting pyruvate was two ...