Y.; MORZHAKOVA, A. A.; GULEVICH, A. Y.; DEBABOV, V. G. Manipulating pyruvate to acetyl-CoA conversion in Escherichia coli for anaerobic succinate biosynthesis from glucose with the yield close to the stoichiometric maximum. Journal of Biotechnology, v. 214, p. 33-42, 2015....
Why is pyruvate converted into acetyl CoA prior to entering the Krebs Cycle? What does this conversion do to the pyruvate molecules? The Krebs Cycle: The Krebs Cycle (also known as the citric acid cycle) refers to the serious of process...
The main purpose of pyruvate oxidation is to oxidize pyruvate to create acetyl CoA. Acetyl CoA is an intermediate of the Krebs cycle. Both pyruvate oxidation and the Krebs cycle are essential components of aerobic respiration, the process of converting food into energy for the cell. How many st...
The pyruvate dehydrogenase complex (PDC) catalyzes the irreversible decarboxylation of pyruvate into acetyl-CoA. PDC deficiency can be caused by alterations in any of the genes encoding its several subunits. The resulting phenotype, though very heterogeneous, mainly affects the central nervous system. ...
The pyruvate dehydrogenase complex (PDHC) catalyzes the irreversible oxidation of pyruvate to acetyl CoA according to the reaction: $$Pyruvate + NA{{D}^{+}}+CoASHo Acetyl CoA + NADH + C{{O}_{2}}$$ The acetyl CoA formed is then either oxidized in the tricarboxylic acid cycle or used ...
Acetyl-CoA was also effective in protecting the enzyme from thermal denaturation. The enzyme was inhibited by 2-oxoglutarate and, to a lesser extent, L-aspartate, with sigmoidal kinetics with respect to acetyl-CoA concentration. The amino acid composition, pH optimum and kinetic constants for ...
The key enzyme for the rTCA cycle, when used as a CO2 fixation pathway, is the ATP-citrate lyase, which catalyzes this reaction: citrate + ATP + CoA → 2-oxosuccinate + acetyl-CoA + ADP + Pi. Heliobacteria lack this enzyme, explaining why they are not autotrophic. It is unclear if ...
The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate to acetyl-CoA. The first component (E1) converts pyruvate to bound acetaldehyde using thiamine diphosphate (ThDP) and Mg2+ as cofactors. There is no 3D structure of E1 available but those of other ThDP-dependent enzymes show...
Under normal conditions, pyruvate dehydrogenase phosphatase activates PDC by reversing PDKs action and further catalyzing pyruvate to acetyl CoA to undergo a process of thecycle. On the other hand,inactivates PDC by phosphorylating at three Ser residues of E1. This causes decreased pyruvate de...
Endogenous pyruvate decarboxylase activity in yeast converts pyruvate to acetaldehyde, which is then converted to ethanol or to acetyl-CoA via acetate (see FIG. 1). Yeasts may have one or more genes encoding pyruvate decarboylase. For example, there is one gene encoding pyruvate decarboxylase in...