We argue that in pea leaf mitochondria the great majority of the acetyl CoA produced by pyruvate oxidation will most likely enter into citrate formation and that the acetyl units will thereby pass into the tricarboxylic-acid cycle. Our data do not indicate any substantial hydrolytic release of ...
EVIDENCE FOR INHIBITION OF ACETYL-COENZYME A FORMATION FROM PYRUVATE IN DIABETIC RAT LIVER. 来自 NCBI 喜欢 0 阅读量: 15 作者:Haft,E David 摘要: HAFT DE.关键词:ACETATES ALLOXAN DIABETES CARBON DIOXIDE CARBON ISOTOPES CHOLESTEROL COENZYME A EXPERIMENTAL LAB STUDY FATTY ACID METABOLISM GLUCOSE ...
1. What is the process by which acetyl-CoA can be formed from an acyl-CoA? Beta-oxidation Beta-mercaptoethylamine Citric acid cycle Pyruvate dehydrogenase cycle 2. What group allows other groups to hang on to the 'tail' of CoA? Sulfhydryl group ...
The purification and assay of these two enzymes, both specific for acyl CoA and the secondary sulfhydryl of (–)-dihydrolipoate, is described. In the assay of lipoic transacetylase (LTA) from E. coli, acetyl-S-lipSH, which resembles acetyl CoA in heat stability and in reactivity with ...
Acetaldehyde production is modulated by NAD+, ATP and acetyl-CoA and is greatly stimulated by lipoic acid. The pyruvate decarboxylase system is extremely sensitive to thiol-group inhibitors and is inhibited by oxygen in the presence of pyruvate. 展开 ...
1. Methods are described for the extraction and assay of acetyl-CoA and of total acid-soluble and total acid-insoluble CoA derivatives in rat epididymal adipose tissue. 2. The concentration ranges of the CoA derivatives in fat pads incub... RM Denton,ML Halperin - 《Biochemical Journal》 被...
Cell-free extracts prepared from cells grown on lactate (without sulfate) contained high activities of methylmalonyl-CoA: pyruvate transacetylase, acetase kinase and reasonably high activities of NAD(P)-independent L(+)-lactate dehydrogenase NAD(P)-independent pyruvate dehydrogenase, phosphotransacetyl...
The synthesis of isocitrate lyase in , the growth of which was stimulated by exogenously added biotin, was released from repression by glucose under biotin-deficient conditions. Biotin deficiency reduced remarkably the levels of biotin-enzymes, pyruvate carboxylase and acetyl-CoA carboxylase, in the ...
The glutaminase enzymes GAC and GLS2 catalyze the hydrolysis of glutamine to glutamate, satisfying the ‘glutamine addiction’ of cancer cells. They are the targets of anti-cancer drugs; however, their mechanisms of activation and catalytic activity have
Pyruvate can be irreversibly channeled into mitochondria by conversion into acetyl-CoA via the multi-enzyme PDH complex that functions as a gatekeeper between glycolysis and mitochondrial respiration. This occurs in particular under aerobic conditions. Pyruvate can also be reversibly converted into lactate...