Y.; MORZHAKOVA, A. A.; GULEVICH, A. Y.; DEBABOV, V. G. Manipulating pyruvate to acetyl-CoA conversion in Escherichia coli for anaerobic succinate biosynthesis from glucose with the yield close to the stoichiometric maximum. Journal of Biotechnology, v. 214, p. 33-42, 2015....
Why is pyruvate converted into acetyl CoA prior to entering the Krebs Cycle? What does this conversion do to the pyruvate molecules? The Krebs Cycle: The Krebs Cycle (also known as the citric acid cycle) refers to the serious of process...
Effects of biotin on pyruvate carboxylase, acetyl-CoA carboxylase, propionyl-CoA carboxylase, and markers for glucose and lipid homeostasis in type 2 diabetic patients and nondiabetic subjects. Survey of the effect of biotin on glycemic control and plasma lipid concentrations in type 1 diabetic patien...
Pyruvate molecules produced by glycolysis are actively transported across the inner mitochondrial membrane, and into the matrix where they can either be oxidized and combined with coenzyme A to form CO2, acetyl-CoA, and NADH, or they can be carbo...
Pyruvate carboxylase (PC) was purified to homogeneity from an overexpressing strain of the purple photosynthetic bacterium Rhodobacter capsulatus using a rapid dye-ligand affinity chromatography procedure, in which dye-bound enzyme was specifically eluted with a low concentration of acetyl-CoA, an alloste...
Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle. In mammalian cells, the pyruvate dehydrogenase co...
Ashman, L. K., Wallace, J. C., and Keech, D. B. (1973) Densitization of pyruvate carboxylase against acetyl-CoA stimulation by chemical modification. Biochem. Biophys. Res. Commun. 51, 924-931.Ashman LK, Wallace JC, Keech DB. Densitization of pyruvate carboxylase against acetyl-CoA ...
When grown with pyruvate, the pyruvate:Fd oxidoreductase (also known as PFOR or pyruvate synthase) likely serves as the major point of input of electrons into the soluble low-potential electron pool and thereby into CEF. During fermentative growth, acetyl-CoA synthetase can convert acetyl-CoA to...
The pyruvate dehydrogenase complex (PDHC) catalyzes the irreversible oxidation of pyruvate to acetyl CoA according to the reaction: $$Pyruvate + NA{{D}^{+}}+CoASHo Acetyl CoA + NADH + C{{O}_{2}}$$ The acetyl CoA formed is then either oxidized in the tricarboxylic acid cycle or used ...
To further complicate the situation, gluconeogenesis is energetically costly, requiring 4 ATP and 2 GTP molecules to convert pyruvate to glucose. Fatty acids and their metabolites, including acetyl coenzyme A (Acetyl CoA), citrate and ketone bodies up-regulate gluconeogenesis particularly in the renal...