Pyrrolysyl-tRNA synthetase (PylRS) is an enzyme of some methanogenic Archaea for the natural incorporation of pyrrolysine into proteins. The discovery of PylRS as a natural tool for genetic code expansion paved the way for site-specific incorporation of non-canonical amino acids (ncAAs) into ...
Pyrrolysyl-tRNA synthetase (PylRS) is a class IIc aminoacyl-tRNA synthetase that is related to phenylalanyl-tRNA synthetase (PheRS). Genetic selection provided PylRS variants with a broad range of specificity for diverse non-canonical amino acids (ncAAs). One variant is a specific phenylalanine...
> Engineering pyrrolysyl-tRNA synthetase for the synthesis :工程吡咯赖氨酰-tRNA合成酶的合成 下载文档 收藏 打印 转格式 20阅读文档大小:1.26M2页dasanma上传于2014-06-18格式:DOC 植物中氨酰tRNA合成酶及相应tRNA的统计分析 热度: 亮氨酸trna及亮氨酰trna合成酶结构和功能的研究 ...
Genetically encoding distinct non-canonical amino acids (ncAAs) into proteins synthesized in cells requires mutually orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs. The pyrrolysyl-tRNA synthetase/PyltRNA pair from Methanosarcina mazei (Mm) has been engineered to incorporate diverse ncAAs and ...
The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNAPyl are extensively used to add non-canonical amino acids (ncAAs) to the genetic code of bacterial and eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process to generate an appropriate PylRS/tRNAPy...
pyrrolysyl-tRNA synthetase genetic code expansion structural basis lysine derivatives translation Introduction The structures and functions of proteins are restricted by the natural variety of building blocks, L-amino acids. Therefore, expanding the repertoire of amino acids in translation is useful for ...
We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNA(CUA Pyl) pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae. In the process we have solved the key challenges of producing a functional tRNA(CUA ...
Pyrrolysyl-tRNA synthetase-tRNAPyl structure reveals the molecular basis of orthogonality. Nature 457, 1163-1167 (2009).Nozawa, K., O'Donoghue, P., Gundllapalli, S., Araiso, Y., Ishitani, R., Umehara, T., Soll, D. and Nureki, O. (2009) Pyrrolysyl-tRNA synthetase- tRNAPyl ...
PYRROLYSYL-TRNA SYNTHETASEObtained is: a method for efficiently producing a polypeptide containing a non-canonical amino acid; a method for incorporating a non-canonical amino acid into a polypeptide; a method for producing tRNA bound to a non-canonical amino acid; a a non-canonical amino acid...
PYRROLYSYL-TRNA SYNTHETASEObtained is: a method for efficiently producing a polypeptide containing a non-naturally-occurring amino acid; a method for introducing a non-naturally-occurring amino acid into a polypeptide; a method for producing tRNA having a non-naturally-occurring amino acid bonded ...