2.慢病毒转染肝癌细胞系SMMC-7721敲除PVT1,并进行基因表达谱芯片及miRNA芯片检测. 3.利用在线靶基因预测软件预测miR-302b-5p靶基因,进行GeneOntology(GO)分析和Pathway分析,并对PVT1敲除后下调基因及miR-302b-5p的靶基因取交集. 结果 1.TCGA数据库数据分析结果显示PVT1在肝癌组织中的表达明显高于癌旁组,且男性...
2018年5月3日,表观遗传大牛张元豪课题组再度在顶级科学期刊Cell上发表题为“Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element”重磅研究,研究人员提出PVT1编码致癌lncRNA,但人类癌症中复发性易位和缺失可能是另一种机制。之前的研究表明PVT1编码致癌lncRNA,但人类癌症中复发性易位和缺失可...
文章来源 Seung Woo Cho, et al. (2018). Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element. Cell 173, 1-15. Reference: 1. Dixon, J.R., Selvaraj, S., Yue, F., Kim, A., Li, Y., Shen, Y., Hu, M., Liu, J.S., and Ren, B. (2012). Topological d...
PVT1 (Plasmacytoma variant translocation 1), a lncRNA encoded by human Pvt1 gene, is located in 8q24 region called "gene desert" . It is involved in several molecular mechanisms: participating in DNA rearrangements, encoding microRNA, interacting with MYC, and regulating its stability. DMs (...
摘要: 目的:分析人浆细胞瘤转化迁移基因(plasmacytomasvariant translocatin 1 gene,PVT1)的rs2720709的A/G多态性与糖尿病肾病(DN)易感性的关系.方法:采取横断面研究方法,选取病程超过5年的本地区汉族住院T2DM患者225例,根据晨尿微量白蛋会议名称: 中华医学会第十二次全国内分泌学学术会议论文汇编 会议时间: 2013...
Here, we show that a single DNA element in the PVT1 gene—the PVT1 promoter—is a tumor-suppressor DNA element that limits MYC oncogene expression. We further show that promoter competition in cis endows PVT1 promoter with the function of a DNA boundary element, blocking MYC oncogene from ...
Ensembl Gene ID- Ensembl mRNA ID- 包装清单: 产品编号产品名称包装 QH21181SHuman PVT1 qPCR Primer Pair1nmol each —说明书1份 保存条件: -20℃保存。建议复溶后进行适当分装,避免反复冻融。 注意事项: PCR扩增产物的长度可能会因基因转录后存在多种剪接形式而有所差异。
Amplification of the 8q24 gene locus is a common event in many malignant diseases and is associated with poor survival rate among patients. Although previous research demonstrates a critical functional role which PVT1 plays in BC, the underlying molecular mechanisms of PVT1 in CL TNBC was ...
The aim of this study was to evaluate MYC and PVT1 copy numbers and gene expressions in early-stage EOC (FIGO I–II) and to assess the biological features and prognostic factors in EOC histological subtypes. Access through your organization Check access to the full text by signing in through...
In addition, in the human pancreatic cancer cell line AsPC-1, functional inactivation of the PVT1 gene can enhance gemcitabine sensitivity, whereas ectopic expression of PVT1 reverses this effect [19]. Collectively, various studies have clearly revealed that PVT1 plays an important role in ...