DMs (Double minutes) is a means of the gene amplification. It carries some oncogenes and drug resistant genes. PVT1 is on the DMs of many cell lines. The study of PVT1 reveals that it is a potential oncogene in many tumors. However, the function of PVT1 still requires much study. ...
Here, we provide evidence that PVT1 may also function as a sponge for miR-20a-5p, as miR-20a-5p levels were not affected following PVT1 knockdown or overexpression. We found that, similar to miR-20a-5p mimics, PVT1 down-regulation was able to suppress the miR-20a-5p target gene ULK1...
Cardiac function, levels of myocardial injury markers and inflammatory indicators were detected following PVT1 knockdown. The regulatory effect of microRNA-143 on PVT1 was assessed using a luciferase reporter gene assay and RT-qPCR analysis. The specific role of PVT1 in regulating the mitogen-...
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Here, we show that a single DNA element in the PVT1 gene—the PVT1 promoter—is a tumor-suppressor DNA element that limits MYC oncogene expression. We further show that promoter competition in cis endows PVT1 promoter with the function of a DNA boundary element, blocking MYC oncogene from ...
Three sh-RNAs against the PVT1 gene were ligated into GV493 vectors, and the components hU6-MCS-CBh-gcGFP-IRES-puromycin (Genechem, Shanghai, China) and plasmid with a nontargeting sequence were used as a negative control (sh-NC). The three target sequences of sh-PVT1 were as follows...
This study investigated the function and mechanism of PVT1 knockdown in the proliferation and malignant transformation of human gliomas. We firstly examined the expression levels of PVT1 and miR-424 in human glioma tissues and cell lines. We also used gene manipulation techniques to explore the ...
We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some...
The interactions among SOX2, lncRNA PVT1, miR-186 and SEMA4D were verified by chromatin immunoprecipitation, RNA immunoprecipitation and dual luciferase reporter gene assay. Gain- and- loss-of-function experiments were conducted to explore the modulatory effects of SOX2, lncRNA PVT1, miR-186 and...
Mitochondrial function and immune response-regulating factor-encoding gene promoters Bidirectional Gene Promoters, 2023, pp. 15-31 Fumiaki Uchiumi C-myc promotes miR-92a-2-5p transcription in rat ovarian granulosa cells after cadmium exposure Toxicology and Applied Pharmacology, Volume 421, 2021, Article...