Protoplasts were prepared from the leaf sheaths of 2-month-old GT42 seedlings. The protoplasts isolated were transfected with the indicated PUC-ScCDPKNT-HA or PUC-ScCDPKFL-HA under 40% polyethylene glycol. After 8 h of incubation, total RNA was isolated and used for cDNA synthesis, followed ...
RNA was pooled from three male mice per diet in experiment 1 and separately from five male and five female mice per diet in experiment 2. For each of these pools, two aliquots of RNA were studied. In experiment 3, RNA from four individual male and four individual female mice per diet ...
The alignment of PyroLuc with 2PSJ shows the secondary structure around the binding pocket of colenteramide to be similar to that of 2PSJ and the colenteramide molecule seems to fit well in the binding pocket (Fig.2). For PyroB there is a shift in the secondary structure as compared to...
RNA was extracted from the knockdown models (n ¼ 2 per cell line), cells expressing a scrambled shRNA, and parental (untreated) cells in triplicate, at independent passages. We tested five PAX8 targeting shRNAs and measured PAX8 expression levels using targeted real-time quantitative PCR ...
MESR3 DNA-binding transcription factor, RNA Pol II-specific X EDS26580.1 – 3.33E−3 1.67 Gadd45 activation of MAPKKK – XP_001652310.1 NP_031862.1 3.63E−7 1.63 CG13868 unknown X EAT33738.1 – 3.49E−6 1.63 Pvr transmembrane receptor protein tyrosine kinase X XP_035905721.1 NP_0010767...
ATP-binding cassette transporter COX2: Cyclooxygenase 2 CD36: Cluster of differentiation 36 GAPDH: Glyceraldehyde 3-phosphate dehydrogenase IL: Interleukin ISI: Insulin sensitivity index LXRα: Liver x receptor alpha MCP-1: Macrophage chemotactic protein-1 PPARγ: Peroxisome proliferator acti...
Preliminary Northern and RNA“dot blot” experiments, as well as Western blots, indicated that Pag is ubiquitously expressed in mammalian cells and tissues, with the highest levels occurring in liver and kidney (not shown). To demonstrate the c-Myc-Pag interaction in mammalian cells, we first ...
(B) The contribution of USP22 to the deubiquitylation activity associated with hSAGA was assessed by using shRNA to deplete USP22 from the FLAG-hGCN5 expressing cells. Control cells were infected with shRNA targeting luciferase (luc). Western blotting confirmed the purification of hGCN5 and ...
where the TSP-1 cDNA fragment was identified via a cDNA subtraction technique and used as a probe, 28S and 18S RNA bands on the membrane were used as control for equal loading; and (c) Northern blot analysis of TSP-1 expression in lung tissue of Runx2 knockout mouse, where glyceraldehyd...
Keywords: cytochrome P450; substrate binding assay; crystal structure; Sphingomonas sp.; X-ray crystallography 1. Introduction Cytochrome P450 enzymes (CYPs) are heme-containing proteins that catalyze the insertion of oxygen into a wide variety of substrates [1,2]. Although many different CYPs have...