Calcineurin B-like CIPK: CBL-interacting protein kinases LUC: Luciferase reporter gene VNTD: Variable N-terminal variable domain AID: Auto-inhibitory junction domain CaM-LD: C-terminal regulatory calmodulin-like domain PAMP: Pathogen-associated molecular pattern NJ: Neighbor-joining Hi-C:...
The alignment of PyroLuc with 2PSJ shows the secondary structure around the binding pocket of colenteramide to be similar to that of 2PSJ and the colenteramide molecule seems to fit well in the binding pocket (Fig.2). For PyroB there is a shift in the secondary structure as compared to...
RNA was pooled from three male mice per diet in experiment 1 and separately from five male and five female mice per diet in experiment 2. For each of these pools, two aliquots of RNA were studied. In experiment 3, RNA from four individual male and four individual female mice per diet ...
Of the 157 PAX8 pathway genes, 154 were profiled on the Illumina HT-12 (three genes not profiled: LUC7L3, PKM, TMA16). Two-sided exact binomial test P-values calculated using the binom.test function in R version 3.0.3 were used to evaluate the proportion of PAX8 pathway genes that ...
MESR3 DNA-binding transcription factor, RNA Pol II-specific X EDS26580.1 – 3.33E−3 1.67 Gadd45 activation of MAPKKK – XP_001652310.1 NP_031862.1 3.63E−7 1.63 CG13868 unknown X EAT33738.1 – 3.49E−6 1.63 Pvr transmembrane receptor protein tyrosine kinase X XP_035905721.1 NP_0010767...
ATP-binding cassette transporter COX2: Cyclooxygenase 2 CD36: Cluster of differentiation 36 GAPDH: Glyceraldehyde 3-phosphate dehydrogenase IL: Interleukin ISI: Insulin sensitivity index LXRα: Liver x receptor alpha MCP-1: Macrophage chemotactic protein-1 PPARγ: Peroxisome proliferator acti...
A Pag cDNA encoding the full-length protein was excised from the original pMyr yeast vector, recloned in pBluescript-SK (Stratagene), and used in a coupled T3 RNA polymerase in vitro transcription/translation reaction in a rabbit reticulocyte lysate system (Promega, Madison, WI). One-tenth ...
Control cells were infected with shRNA targeting luciferase (luc). Western blotting confirmed the purification of hGCN5 and the knockdown of endogenous USP22 within purified hSAGA (upper panels). Efficient USP22 depletion was also documented at the mRNA level by qRT-PCR (middle panel). hSAGA ...
where the TSP-1 cDNA fragment was identified via a cDNA subtraction technique and used as a probe, 28S and 18S RNA bands on the membrane were used as control for equal loading; and (c) Northern blot analysis of TSP-1 expression in lung tissue of Runx2 knockout mouse, where glyceraldehyd...
Comparison with other CYP structures suggested that conformational changes in the β1–β2, α3–α4, and α6–α7 connecting loop are important for incorporating the long hydrophobic alkane-like substrate. These results improve the understanding of the catalytic mechanism of CYP153D17 and provide...