c Protein levels of ScCDPK1NT, ScCDPK1FL, ScCDPK8NT, and ScCDPK8FL as indicated in sugarcane protoplasts. Protoplasts isolated from Guitang 42 were transfected with Pro35s:ScCDPK1 FL-HA, Pro35s:ScCDPK8 FL-HA, Pro35s:ScCDPK1 NT-HA, or Pro35s:ScCDPK8.NT -HA. Total RNA was isolated...
Total RNA was then subjected to RNeasy Cleanup (Qiagen) for microarray and Q-PCR. For microarrays, 20 μg of total RNA was reverse transcribed using Superscript II (Invitrogen) and a poly-dT primer containing the T7 RNA polymerase binding site (Genset Corporation). Second-strand cDNA was ...
The alignment of PyroLuc with 2PSJ shows the secondary structure around the binding pocket of colenteramide to be similar to that of 2PSJ and the colenteramide molecule seems to fit well in the binding pocket (Fig.2). For PyroB there is a shift in the secondary structure as compared to...
MESR3 DNA-binding transcription factor, RNA Pol II-specific X EDS26580.1 – 3.33E−3 1.67 Gadd45 activation of MAPKKK – XP_001652310.1 NP_031862.1 3.63E−7 1.63 CG13868 unknown X EAT33738.1 – 3.49E−6 1.63 Pvr transmembrane receptor protein tyrosine kinase X XP_035905721.1 NP_0010767...
For differential expression, P-values from two-tailed t-tests, false discovery rate (FDR) by the Benjamini-Hochberg method, and fold changes were calculated for the following comparisons: HeyA8 untreated plus scrambled controls vs HeyA8 treated with PAX8 shRNA-1 and shRNA-2 and luciferase-...
ATP-binding cassette transporter COX2: Cyclooxygenase 2 CD36: Cluster of differentiation 36 GAPDH: Glyceraldehyde 3-phosphate dehydrogenase IL: Interleukin ISI: Insulin sensitivity index LXRα: Liver x receptor alpha MCP-1: Macrophage chemotactic protein-1 PPARγ: Peroxisome proliferator acti...
A Pag cDNA encoding the full-length protein was excised from the original pMyr yeast vector, recloned in pBluescript-SK (Stratagene), and used in a coupled T3 RNA polymerase in vitro transcription/translation reaction in a rabbit reticulocyte lysate system (Promega, Madison, WI). One-tenth ...
(A) H1299 cells were treated with shRNA directed against c-myc. Myc protein levels were assessed by western blotting (left panel), and qRT-PCR was used to define levels of the Myc target genes CAD and MTA1 (right panel). (B and C) Within the CAD and MTA1 loci, binding of endogen...
where the TSP-1 cDNA fragment was identified via a cDNA subtraction technique and used as a probe, 28S and 18S RNA bands on the membrane were used as control for equal loading; and (c) Northern blot analysis of TSP-1 expression in lung tissue of Runx2 knockout mouse, where glyceraldehyd...
These results improve the understanding of the catalytic mechanism of CYP153D17 and provide valuable information for future protein engineering studies. Keywords: cytochrome P450; substrate binding assay; crystal structure; Sphingomonas sp.; X-ray crystallography 1. Introduction Cytochrome P450 enzymes (...