Inhibition by Puromycin of Incorporation of Tritiated Uridine into Nucleolar and Cytoplasmic Ribonucleic AcidsPUROMYCIN is a specific inhibitor of protein synthesis in bacterial and mammalian cellsand can therefore be used as a tool for investigating the dependence of various processes on concomitant ...
它允许通过免疫荧光显微镜直接评估单个细胞中的翻译活性，以及通过荧光激活细胞分选法直接评估异质细胞群中的翻译活性[3]。参考文献：[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92.[2]. M...
the middle lane is from cells incubated for 2 hr in medium lacking glucose and serum, and the last lane is cells incubated for 1.5 hr without glucose or serum and then glucose and serum were returned during the last 30 min. As expected, puromycin incorporation was lower in the immobilized ...
参考文献: [1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92. [2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids...
Nathans, D.: Puromycin inhibition of protein synthesis: The incorporation of puromycin into peptide chains. Proc. Natl. Acad. Sci. U.S. 51, 585 (1964). Article CAS Google Scholar Nathans, D.: Cell-free protein synthesis directed by coliphage MS2 RNA. J. Mol. Biol. 13, 521 (1965)....
Inhibition of protein synthesis by puromycin stops the incorporation of glucosamine into Bel-infected HeLa cells almost immediately, suggesting that glycosylation... P Stanley,SS Gandhi,DO White - 《Virology》 被引量: 146发表: 1973年 The role of ascorbic acid in the biosynthesis of collagen II. ...
Related Terms Chapters and Articles You might find these chapters and articles relevant to this topic. Chapter Puromycin Encyclopedia of Toxicology (Fourth Edition) Reference work2024,Encyclopedia of Toxicology (Fourth Edition) RebekahTorchon,Madan K.Kharel ...
[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92. [2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 200...
产品活性:Puromycin dihydrochloride (CL13900 dihydrochloride) 是一种氨基核苷类抗生素,抑制蛋白合成 (protein synthesis)。 IC50 & Target:Aminoglycoside 参考文献: [1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. ...
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by...