pulse-chase labeling实验原理Pulse-chase labeling实验原理是通过给予细胞标记性核苷酸脉冲,然后在 chase 阶段监测和分析细胞内标记的分布和保留,以研究细胞周期、蛋白质合成和半衰期等生物学过程。©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | 网站地图 | 百度营销 ...
Pulse-chase experiments have proved to be a powerful tool to study protein folding, maturation, and degradation in mammalian cells. When short pulses are applied, a fraction of the total protein pool can be followed from synthesis to degradation in its natural environment.DOI...
Pulse-chase experiments allow study of the fate of proteins after synthesis, such as processing, intracellular transport, secretion, degradation, and physical chemical properties of proteins. Pulse-chase protocols have been used to analyze the phosphorylation of endogenous and transiently expressed protein...
Radioactive pulse chase studies concerning the synthesis of viral proteins : and the membrane assembly of Semliki forest virus Administration of Semliki Forest virus system; Effect of tunicamycin on receptor maturation; Details on the pulse-chase labeling experiments in BHK-21 cells... CD Richardson...
网络脉冲追踪标记 网络释义 1. 脉冲追踪标记 ...nactivation)、检测蛋白质合成过程、进行脉冲追踪标记(pulse-chase labeling)以及电镜下定位等等。 www.lifeomics.com|基于 1 个网页
Using optical pulse-chase labeling (OPL) methodology we identified that both soluble and insoluble, phosphorylated WT and mutant A53T human α-syn, show similar rates of production and turnover to Dendra2 alone. However, upon addition of α-syn PFFs some neurons show a slowing of α-syn ...
Analysis of Protein Turnover by Quantitative SNAP‐Based Pulse‐Chase Imaging Assessment of protein dynamics in living cells is crucial for understanding their biological properties and functions. The SNAP-tag, a self labeling suicid... DL Bodor,MG Rodríguez,N Moreno,... - 《Curr Protoc Cell ...
13CO2pulse-chase experiments monitored by high-resolution NMR spectroscopy and mass spectrometry can provide13C-isotopologue compositions in biosynthetic products. Experiments with a variety of plant species have documented that the isotopologue profiles generated with13CO2pulse-chase labeling are directly com...
A monomeric fluorescent protein that can be irreversibly photoswitched from green to red form, both of which can be reversibly photoactivated, is reported. It is applied to pulse-chase experiments in which dynamic structures in live cells are imaged with
This is particularly relevant in cases where an individual protein might not be stably bound within a complex, but might be readily exchangeable, as has been noted in radioactive labeling experiments (5). To differentiate between these two possibilities, we conducted pulse-chase SILAC experiments ...