Using human kidney organoids, primary endothelial cells, and patient samples, we demonstrate that IFN-γ induces pyroptotic angiopathy in combination with APOL1 expression. Single-cell RNA sequencing, immunoblotting, and quantitative fluorescence-based assays reveal that IFN-γ-mediated expression of ...
Survival time was measured from time of transplantation until time mice had to be culled due to overt clinical symptoms. Bone marrow tissue was harvested by dissection of the leg bones of the mouse before the bones were flushed with PBS to obtain the cells. After collection 2 ml of red ...