The invention discloses a plasmid expressed by prostate-specific antigen (PSA) promoter mediated firefly luciferase gene. The plasmid is formed by connecting a PSA promoter pPSA-ZsGreen dominant skeleton with a firefly luciferase gene fragment. The plasmid is applied to the transfection of prostate ...
Cells stably infected with the luciferase construct were selected in hygromycin (1 mg/mL) containing medium. Cell models for expressing Wt and PSA variants PSA constructs generated as described above were transfected into PC-3 (do not express PSA), LNCaP (high PSA expression) and patient-...
PSA expression was examined by RT-PCR and western blot and PSA promoter activity by luciferase reporter assay. Results In normal prostate C/EBPα was expressed in the basal layer of the epithelium. In prostate cancer C/EBPα was detected at low levels throughout the cancers and in advanced ...
Also, inhibition of PSA expression by telmisartan was confirmed by western blot and luciferase assay, indicating that an ARB acted in a similar way such as an anti-androgenic agent in prostate cancer cells. CONCLUSION The present study showed ARBs, especially those possessing a PPAR ligand-like ...
如您需要在细胞上做基因编辑,荧光标记等操作,本株细胞有对应的报告基因细胞系,现货有Cas9/CRISPRi稳定持续表达和诱导表达,以及Luciferase示踪细胞和单细胞分辨率的示踪细胞等...其他编辑需要,可以联系销售负责 做定制需求。 细胞来源:国外细胞库(有COA,STR)。传代3代以内(小于等于三)。100%可溯源。 公司产品齐全,如...
The molecular mechanisms involved in mediating the effect of PSA on bone cells were examined by the use of TGFβ-responsive luciferase reporter assays and siRNA against Wnt-inhibitory factor-1 (Wif-1). Here we probed further into the modulation of osteogenic gene expression by transfection of ...
lentivirus vector plasmid with pVSV-G (expressing the VSV envelope protein) pREV and pRRE and (expressing lentivirus helper functions), as described previously.25 Prostate cancer cells were transduced with lentiviruses expressing shRNAs directed against either luciferase or GFP (as a control) or MAP...
本发明公开了一种前列腺特异性抗原(PSA)启动子介导荧光素酶基因表达的质粒.该质粒由PSA启动子pPSA-ZsGreen显性骨架和荧光素酶Firefly Luciferase基因片段连接而成.将质粒转染前列腺癌细胞,为构建前列腺癌的PSA荧光报告基因动物模型奠定基础.通过荧光定位前列腺癌动物模型中肿瘤的部位,可为前列腺癌的治疗和新型药物的研发...
Knockdown of PABPC1 with siRNA decreases PSA luciferase activity and PSA protein levels.Kurtis EisermannJavid A. DarJun DongDan WangKhalid Z. MasoodiZhou Wang
PSA expression was examined by RT-PCR and western blot and PSA promoter activity by luciferase reporter assay. Results In normal prostate C/EBPα was expressed in the basal layer of the epithelium. In prostate cancer C/EBPα was detected at low levels throughout the cancers and in advanced ...