It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same...
(bp 4982412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3 f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla...
promoter: lacpromoter for in vitro transcription: T3promoter for in vitro transcription: T7replicon: ARSH4replicon: f1replicon: pMB1MCS: SacI...KpnIcentromere: CEN6 Applications YC-type (centromeric) shuttle vector plasmid shuffling vector containing primer sites useful for sequencing vector permitting...