Proximity labeling (PL) is an alternative approach to bio-orthogonal labeling. Genetically encoded labeling enzymes such as BioID10, TurboID11, and APEX212can be expressed and localized to a specific subcellular compartment. In the presence of exogenous biotin substrates, in situ biotinylation occurs ...
To quantify the relative abundance of PL proteins, we prepared and analyzed Group I samples without p50 and Group II samples with p50 (Fig.2b). TurboID labeling was performed for 12 h by infiltration of biotin. Expression of tRFP-p50 was induced by the addition of estradiol at the same ...
In this paper, we review a broad range of proximity labeling techniques, their development, drawbacks and advantages, and implementations in recent studies.doi:10.1002/asia.202101240Olha ShkelConvergence Research Center for Diagnosis Treatment and Care System of Dementia Korea Institute of Science and ...
Over the past decade, photocatalytic proximity labeling has emerged as one of the most powerful and versatile techniques for studying these interactions as well as uncovering subcellular trafficking patterns, drug mechanisms of action, and basic cellular physiology. In this article, we review the basic...
To further characterize ANXA3 function in HCV, we performed proximity labeling using ANXA3-BioID2 as bait in HCV-infected cells. Two of the top proteins identified proximal to ANXA3 during HCV infection were the La-related protein 1 (LARP1) and the ADP ribosylation factor-like protein 8B (...
To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different cell types identified the vast majority of previously validated LD proteins, excluded common contaminating ...
Integrated Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Quantitative Proteomic Analysis Identifies Galectin-1 as a Potential Biomarker for Predicting Sora... Sorafenib has become the standard therapy for patients with adv...
Keywords TurboID Proximity Labeling Protein protein Interactions NLR Immune Receptors Nicotiana Benthamiana Agroinfiltration Biotin RIPA Lysis Buffer Desalting Column Play Video Authors Overview Publishing Process Editorial Board Scope and Policies Peer Review ...
In this paper, we review our recent development and validation of the ultrasensitive electronic biomolecular assays enabled by our novel amplifying nanowir... OC Chi,KS Shin,Y Mao - Spie Conference on Nanoepitaxy: Materials & Devices 被引量: 0发表: 2013年 加载更多来源...
a Identification of proteins that are specifically associated with SUN1 at the INM using proximity labeling. The ONM protein WIT1 serves as a control to exclude proteins associated with both the INM and the ONM. b Pairwise ratiometric analyses of PL-LFQMS data using 35S: HA-BioID2-SUN1 ...