Department of Chemistry, Stanford University, Stanford, CA, USA Alice Y. Ting Chan Zuckerberg Biohub, San Francisco, CA, USA Alice Y. Ting Contributions K.F.C., T.C.B, N.D.U., S.A.M., S.A.C., and A.Y.T. contributed to the writing and editing of the manuscript. ...
In situ profiling of subcellular proteomics in primary living systems, such as native tissues or clinic samples, is crucial for understanding life processes and diseases, yet challenging due to methodological obstacles. Here we report CAT-S, a bioorthogonal photocatalytic chemistry-enabled proximity label...
Bioorthogonal chemistryDNA-templated chemistryFluorogenic probesInverse electron demand Diels-Alder reactionBioorthogonal chemistry techniques enable the selective and targeted manipulation of living systems. In order to yield universally applicable techniques, it is of great importance for bioorthogonal......
Journal of Biological Chemistry, Volume 300, Issue 5, 2024, Article 107290 Ankanahalli N. Nanjaraj Urs,…, Hani S. Zaher View PDF The molecular determinants of classical pathway complement inhibition by OspEF-related proteins of Borrelia burgdorferi Journal of Biological Chemistry, Volume 300, Is...
Lobingier, B. T. et al. An approach to spatiotemporally resolve protein interaction networks in living cells.Cell169, 350–360.e12 (2017).This study utilized APEX to map the dynamic interactomes of the δ-opioid receptor, a GPCR, in response to agonist activation. ...
A Bifunctional Fluorogenic Rhodamine Probe for Proximity-Induced Bioorthogonal ChemistrydienophileskineticsproteinsrhodaminetetrazineBioorthogonal reactions have emerged as a versatile tool in life sciences. The inverse electron demand Diels- Alder reaction (DA_(inv)) stands out due to the availability of ...
we turned to light-induced LLPS systems. Recently, light-induced protein clustering was successfully used to temporally trigger and analyze IDR phase separation behaviors in living cells29,30,31. We used a light-activatable protein CRY2, which undergoes oligomerization in response to a 488 nm ...
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein–protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotinylation ...
The combination of addressable DNA assembly and multienzyme cascades can deliver breakthroughs toward the engineering of novel synthetic and biomimetic reactors.doi:10.1007/s41061-020-0299-3Jinglin FuZhicheng WangXiao Hua LiangSung Won OhTing ZhangSpringer International PublishingTopics in Current Chemistry...
Nucleotide-binding leucine-rich repeat (NLR) immune receptors play a critical role in defence against pathogens in plants and animals. However, we know very little about NLR-interacting proteins and the mechanisms that regulate NLR levels. Here, we used