Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in response to blue light irradiation. When fused to various RNA effectors, LicV allows for optogenetic control of RNA localization, splicing, translation and stability in cell culture. Further...
The core protein has three domains: (i) the 140 amino-acid (aa) N-terminal domain (NTD), mostly structured into an alpha-helical domain known to be involved in capsid assembly10,11; (ii) a linker formed by residues 141–149, of unknown function but potentially involved in the regulation...
A plant lectin derived from Viscum album induces cytokine gene expression and protein production in cultures of human peripheral blood mononuclear cells. These results suggest that lectin-sugar interactions on the cell surface of immunocompetent cells can induce cytokine gene expression and protein synthes...
Recent years have witnessed dramatic advances in our understanding of how newly translated proteins fold in the cell and the contribution of molecular chaperones to this process. Folding in the cell must be achieved in a highly crowded m... J Frydman - 《Annual Review of Biochemistry》 被引量...
In vivo and in cultures, HCRPs antibodies label neuronal soma and neurite extensions, giving the appearance of both cytoplasmic and cell surface immunostaining. On the other hand, no expression is found on nonneural tissues. Functionally, HCRPs are involved in neurite growth control and appear to...
These events typically rely upon a series of protein–ligand and protein–protein interactions. Such signals must effectively propagate across long distances and even across barriers, such as the cell membrane. Each step in the signaling pathway must be highly specific, both to preserve the ...
A total of 106 differentially-expressed proteins were identified during the normal, model and administration group. Most of them participate cellular processes. Adherens junction and focal adhesion pathways play a regulatory role in various cell signaling pathways. Protein ADRM1 was validated. Compared ...
Microsporidian polar tube proteins: Highly divergent but closely linked genes encode PTP1 and PTP2 in members of the evolutionarily distant Antonospora and... The spore polar tube is a unique organelle required for cell invasion by fungi-related microsporidian parasites. Two major polar tube protei...
Of gene expression and cell division time: a mathematical framework for advanced differential gene expression and data analysis. Cell Syst. 9, 569–579 (2019). This study formalizes the role of cell cycle time in the context of gene expression. CAS PubMed Google Scholar Wilhelm, M. et al...
Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking. Mol. Cell Biol. 18, 5308–5319 (1998). Article CAS PubMed PubMed Central Google Scholar Itakura, E. & Mizushima, N. Syntaxin 17: the autophagosomal SNARE. Autophagy ...