whereas GTP-γ-S impairs XLG2–AGB1 interaction. The indicated constructs were transfected into Col-0 protoplasts, and co-IP assays were performed in the presence of the indicated nucleotides. Note that endogenous AGGs are likely essential for the interaction. Numbers indicate ...
3c). These results confirm that the nAPH and TM1 of Yop1p are dispensable for tubule formation. We also purified the cytL-to-GS mutant and reconstituted it into proteoliposomes (Supplementary Fig. 4c, d). Within 4.5 h of detergent removal, short tubules were seen by TEM (Supplementary ...
and play fundamental roles in RNA regulation. However, in the human malaria parasite,Plasmodium falciparum, identification and characterization of these proteins are particularly limited. In this study, we use an unbiased proteome-wide approach, called R-DeeP, a method based ...
All the protease enzymes that are released in the stomach and the intestine are released in an inactive state or in the form of proenzyme. The proenzyme is converted to an active enzyme with the help of specific pH or the action of anothe...
The phthalimide (53)-based conjugated ligands have been widely used to develop the libraries of CRBN-targeting PROTACs, which can be easily converted into multiple PROTAC precursors196. Recently, several CRBN modulators have been reported, which were demonstrated to mediate antitumor effects through ...
In the first step the scleroproteins are loosened up by agents, capable of splitting up peptide bonds, in such a manner that they are still water-insoluble but have become digestible, whereas in the second step they are converted by enzymatic decomposition into water-soluble compounds. Both ...
During succinylation, net positive charges are replaced with a net negative charge at hydroxyl and lysine amino group of proteins, whereas in acetylation basic groups are converted into neutral ones (Aryee et al., 2018, pp. 27–45). The surface charge of plant-based proteins changes after ...
genes expressed in liver and heart can be identified using the structure selection tool of the Genepaint database [4]. Images of individual sections of each set retrieved can be inspected using image viewers that are integrated into the database. For additional instructions on how to access Genep...
Small RNAs derived from specific genes; the transcripts are cleaved by specialized miRNAs and the cleavage products can be converted into double-stranded RNAs by RNA-dependent RNA polymerases. A Dicer enzyme cleaves the double stranded RNA to ta-siRNAs. ta-siRNAs are involved in DNA methylation. ...
Can be converted into other energy units such as calories, joules or electronvolts. Lipid mixing Mixing of membrane lipids during fusion, often describing the stage when the contacting lipid leaflets have mixed and the two distal leaflets remain separated. Secretory pathway A system that connects ...