we need to develop technologies that simultaneously deliver multiple gRNAs and base editors protein into a single mammalian cell for TAG to TAA recoding. With the advancements in DNA synthesis capability and exponential cost reduction, we directly designed and synthesized gBlocks containing five individual...
necessary for BER. A possible function for the second Wing domain is to interact with other repair enzymes. Protein–protein interactions are thought to enhance repair efficiency by assuring transfer of DNA intermediates between enzymes in the repair pathway and preventing inappropriate DNA transactions8...
$ biomojify --help usage: biomojify [-h] [--version] [--log LOG_FILE] {fasta,fastq} ... Read one or more FASTA files, and convert them to emoji.😀 positional arguments: {fasta,fasta_protein,fastq,vcf} sub-command help fasta fasta --help fasta_protein fasta_protein --help fastq...
Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation Tet proteins can convert 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) through three consecutive ... H Wu,Y Zhang - 《Genes & Development》 被引量: 787发表: 2011年...
DNA.ConsensusSequencce to be invoked through Transform as followsvar orfs = dnaSeq.FindORFs(minLength: 15, maxLength: 150); var seq = orfs.FirstOrDefault().CastTo<Protein>().Transform<ConsensusSequence, string>();Or alternatively invoking Transform weakly-typed:var...
Alternative splicing converts the G-protein coupled follitropin receptor gene into a growth factor type I receptor: implications for pleiotropic actions of the hormone. Mol Reprod Dev 1997; 48:471-479.Sairam MR, Jiang LG, Yarney TA & Khan H 1997 Alternative splicing converts the G-protein ...
Converting bam to bigwig and separating according to strand could be useful to visualize nucleosomal or protein binding 'footprints' in ChIP-Seq experiments as at the boundaries of protein-DNA interactions will be marked with reads mapping on different DNA strands. 展开 ...
鱼类PKZ(Protein Kinase Containing Z-DNA Binding Domain)Zα与Z-DNA的结合 真核细胞翻译起始因子2α(eIF2α)激酶是在进化中高度保守的丝氨酸/苏氨酸蛋白激酶,存在于哺乳类不同的组织和细胞中,通过调节eIF2α的活性参与机体内多种应激反应。新... 吴初新 - 《南昌大学》 被引量: 0发表: 2010年 加载更多来...
A researcher developed a method to convert mRNA of any species into a CRISPR guide RNA library using a semi-random primer. The clustered regularly interspersed palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9) system is a powerful tool for genome editing that can be used to constru...
Our studies suggest that mtDNA repair is performed by long-patch BER that provides an optimal substrate for Pol g when fulfilling its role in DNA repair. Results Refolding and purification of hEXOG. hEXOG is a mitochon- drial membrane protein19, and the hEXOG construct used here lacks the ...