The CSF of all individuals was analyzed for 63 brain enriched proteins using a method of liquid-chromatography tandem mass spectrometry. Wilcoxon rank sum test, Kruskal-Wallis one-way ANOVA, logistic regression, and Pearson correlation were used to refine the list of candidates by comparing relative...
Identification of a Meningococcal L-Glutamate ABC Transporter Operon Essential for Growth in Low-Sodium Environments Tautomerism and resonance-assisted hydrogen bonding have been analyzed on the basis of the results of abinitio calculations at the MP2/AUG-cc-pVDZ level of... C.,Monaco,A.,... ...
Detection of CSF 14-3-3 protein in Guillain–Barré syndrome CSF samples from patients with GBS, chronic neuropathies, motor neuron disease (MND), definite sporadic Creutzfeldt–Jakob disease (sCJD), and normal control subjects were analyzed by standard immunoblot assay, using a polyclonal anti-14...
Cell membrane protein (CMP) profile of HIV-1 from cerebrospinal fluid (CSF) and plasma of five AIDS patients with neurologic disorders was analyzed and compared with viral quasispecies composition in these body compartments. To this aim, paired CSF and plasma samples from AIDS subjects with HIV-...
Purified proteins are stored in animal-free containers in a dedicated cold storage room. Quality Assurance Endotoxin Level: < 1.0 EU per 1 µg of product. No impairment of biological activity. High quality product obtained under stringent conditions. ...
CSF specimens were analyzed for concentrations of SP-A, SP-C, and SP-D by using enzyme-linked immunosorbent assays (ELISAs). Axial T2w turbo inversion recovery magnitude (TIRM) magnetic resonance imaging was employed in all cases. Using a custom-made MATLAB-based tool for quantification of ...
The recent research progress of recombinant protein refolding was reviewed.This paper analyzed various kinds of solution-phase and solid-phase refolding technologies,proposed the refolding optimization procedure and introduced the new solution of physical refolding such as high pressure refolding based upon ...
f, hPD-L1-Fc binding to GFP-tagged RPL20B in S. cerevisiae lysate analyzed by microscale thermophoresis with biological triplicates. g, Binding intensity of hPD-L1-Fc to different fungal RPL20B homologs displayed on yeast. Each circle represents one yeast colony (n = 3). h, Schematic ...
Kussmann M & Roepstorff P (2000). Sample preparation techniques for peptides and proteins analyzed by MALDI-MS. Meth Mol Biol 146, 405–424. Leitner A & Lindner W (2004). Current chemical tagging strategies for proteome analysis by mass spectrometry. J Chromatogr B Anal Technol Biomed Life...
To define the expression pattern of members of the JAK/STAT pathway during normal granulopoieses, we analyzed the proteins in normal myeloid cells at different stages of maturation. These data were compared with the expression pattern of these proteins in leukemic cells from 25 patients and 12 ...