抗体凝集实验是指颗粒性抗原与相应抗体结合后发生凝集的血清学试验。抗原与抗体复合物在电解质作用下，经过一定时间，形成肉眼可见的凝集团块。试验可在玻板上进行，称为玻板凝集试验，可用于细菌的鉴定和抗体的定性检测；亦可在试管中进行，称试管凝集试验，主要用于抗血清效价测定。玻片法是一种定性试验方...
蛋白Z抗体ProteinZ免疫标记,Western Blot抗体杂交: 孵育一抗 A. 先将需要检测的抗体准备好,并决定好它们的稀释度。 B. 配好5%的Milk(TBST溶解),按要求稀释好抗体。注意,如需高比例稀释, 采用梯度稀释。 C. 将稀释好的抗体和膜一起孵育。 一般采用RT 1小时,可根据抗体量和膜上 ...
Enzyme-linked immunosorbent assays (ELISAs) are a common test to quantify antibody or antigen binding activity. For more detail, see our ELISA page. Read more about our ELISA Assay Development Western Blot The Western blot is a standard technique used to quantify the amount of target protein pr...
Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗。经过PAGE分离的蛋白质样品,转移到固相载体(例如硝酸纤维素薄膜)上,固相载体以非共价键形式吸附蛋白质,且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原,与对应的抗体起免疫反应,再...
The extracted protein was tested in a western blot analysis using an Anti-His antibody, showing all three colonies (1–3; L = molecular weight ladder) produced the expected size recombinant protein, with the highest yield in colony 1 which was produced in 1 L for the Calcium ...
Ida, N.et al.Analysis of heterogeneous A4 peptides in human cerebrospinal fluid and blood by a newly developed sensitive Western blot assay.J. Biol. Chem.271, 22908–22914 (1996). ArticleCASGoogle Scholar Rose, C.et al.New highly sensitive rodent and human tests for soluble amyloid precursor...
Cell or EV samples were quantified and compared side by side with western blot and TotalSeq-A antibody assays, and results showed that the two approaches were comparable (Fig. 1e,f). However, the TotalSeq method is time and sample saving, and has a greater potential for multiplex detection...
western blot.cDetection of ZIKV proteins in captured sterol–protein complexes by western blot using antibodies specified on the right. Molecular weights are indicated on the left (in kilodalton; kDa). A representative result is shown (n = 4). Samples without UV irradiation but otherwise ...
The neutralization test showed that 3B7 neutralized the DHAV-1 HP1 virus with a neutralization titer of 16. 4F8 and 3E9 did not show any neutralizing activity. VP3 protein detection by Western blot To prove VP3 expressed in pCI-neo-VP3 transfected DEF cells, duck anti-DHAV-1 sera were...
(C) Same as (A and B), but for albumin Western blot. (D) Comparison of the untransformed values obtained by each method over the range of 0.25–20 μg total protein loading. Data are shown as the mean ± SD for triplicate experiments using separate pooled samples. We also evaluated ...