1. 蛋白质翻译 蛋白质翻译(Protein Translation)细胞因子酶联免疫斑点检测(The Cytokines ELISPOT Assay ) 蛋白芯片(Protein chip) RT-PCR(… ndklgs.lzu.edu.cn|基于5个网页 2. 蛋白质转译 ...DNA 序列转成蛋白质序列 (Expasy) 1.蛋白质转译(Protein translation) • 先连到 ExPASY的网站 - http://tw...
Recently, a single, centralized, authoritative resource for protein sequences and functional information, UniProt, was created by joining the information contained in Swiss-Prot, Translation of the EMBL nucleotide sequence (TrEMBL), and the Protein Information Resource–Protein Sequence Database (PIR–...
Thesecondstrategy is based on a hypothesis that proteins located in different subcellular localizations do not interact. A protein can be divided into seven groups based on subcellular location information extracted from Swiss-Prot (http://www.expasy.org/sprot/), including cytoplasm, nucleus, mitochon...
TrEMBL, which stand for “Translation of the EMBL nucleotide sequences”, was created in 1996 in order to cope with the increasing data flow produced by the genome projects. Every CDS proposed by the submitter of a nucleic acid sequence deposited either at EMBL, GenBank or DDBJ is automatica...
Energy Signaling in the Regulation of Gene Expression during Stress Baena-GonzálezElena, inMolecular Plant, 2010 Ribosomal Proteins Another layer of complexity lies onribosomal proteins, which are encoded by small gene families whose translation is strongly repressed by mild dehydration stress, for examp...
proteindatabasessequence蛋白质序列expasygenomes Marie-Claude.Blatter@isb-sib.ch Swiss-Prot group, Geneva SIB Swiss Institute of Bioinformatics Protein sequence databases http://education.expasy/cours/UniProt/ Menu Introduction Nucleic acid sequence databases ENA, GenBank, DDBJ Protein sequence databases UniP...
The isoelectric point was calculated with ExPASy (https://web.expasy.org/compute_pi/) and the grand average of hydropathy (GRAVY) was calculated with the online sequence manipulation tool (http://www.bioinformatics.org/sms2/protein_gravy.html). For disorder prediction, IUPred56 was used with ...
Knowledge of a protein's isoelectric point can aid in determining the most appropriate type of ion exchange chromatography. Online tools are available for calculating the theoretical pI of a proteinEXPASY. These calculations are based entirely on the amino acid sequence of a protein and do not tak...
Therefore, the translation of spliced and active Xbp1 mRNA may be attenuated by the PERK-eIF2α pathway. In this study, we revealed that pXbp1 suppression is involved ER-mediated cell death through the regulation of target gene expression, such as ERdj4, p58IPK, and BiP. In addition, the...
Protein concentration was determined using an ND-1000 spectrophotometer (Thermo Scientific) at 280 nm with theoretical extinction coefficients as determined by the Expasy ProtParam tool as shown in Table S2, in the case of T14-3-3. In the case of the His-SUMO-c-Raf S233/S259 variants, ...