The protein composition of CNS myelin is simpler than that of other brain membranes, with the myelin basic protein (MBP) and proteolipid protein (PLP) making up 60 to 80% of the total in most species. Many other proteins and glycoproteins are present to lesser extents. With the exception of...
2.6CPT1 activity The substrates were palmitoyl-CoA andcarnitine. In theradiometricassayl-(methyl-3H) carnitine (Amersham Bioscience, USA) was used.Enzyme activitywas assayed for 4min at 30°C in a total volume of 200μL. The protein sample, 40μL (20–40μg), was pre-incubated for 1mi...
Protein acetylation has a crucial role in energy metabolism. Here we performed the first large-scale profiling of acetylome in rat islets, showing that almost all enzymes in core metabolic pathways related to insulin secretion were acetylated. Label-free
Acbp6 function tunes acetyl-CoA metabolism and protein acetylation to regulate midgut tissue plasticity during nutrition adaptation We hypothesized that aberrant ‘switching’ of metabolic networks in the absence of Acbp6 is likely to influence more than just energy output from mitochondria during nutrient...
DNA-protein crosslinks (DPCs) are toxic lesions that inhibit DNA related processes. Post-translational modifications (PTMs), including SUMOylation and ubiquitylation, play a central role in DPC resolution, but whether other PTMs are also involved remains
Inhibition of AMPK is tightly associated with metabolic perturbations upon over nutrition, yet the molecular mechanisms underlying are not clear. Here, we demonstrate the serine/threonine-protein phosphatase 6 regulatory subunit 3, SAPS3, is a negative r
Increasing evidence highlights the potential role of long non-coding RNAs (lncRNAs) in the biological behaviors of renal cell carcinoma (RCC). Here, we explored the mechanism of AGAP2-AS1 in the occurrence and development of clear cell RCC (ccRCC) involv
Individual responses to oxaliplatin (L-OHP)-based chemotherapy remain unpredictable. The objective of our study was to find candidate protein markers for tumor sensitivity to L-OHP from intracellular proteins of human colorectal cancer (CRC) cell lines.
(b) Schematic representations of a typical CCN gene and protein. Each CCN gene consists of five exons, each of which encodes a module in the protein. IGFBP, insulin-like growth factor binding protein; VWC, von Willebrand factor type C repeat; TSP1, thrombospondin-1 or TSR repeat. All CCN...
and their role in mating, RNA-seq was carried out on the testes of maleC. pomonellaat two developmental stages: the second day (presexual maturity, CpT2D) and the fifth day (postsexual maturity, CpT5D). The entire male body, excluding the testes, served as the control groups (CpT2DC...