Application Note: Protein Thermal Shift Technology Data Sheet: Protein Thermal Shift Dye 有限使用商标许可 ' Notice to Purchaser: The purchase of this Applied Biosystems Melt Analysis Software provides the limited, non-transferable right for the purchaser to perform Life Technologies' patented Protein The...
Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。 节省样品筛查所花费的时间和费用 与现有方法相比,这一解决方案可大幅减少样品筛选相关...
As a result, we developed the Protein ThermalShiftTM software v1.0 which utilizes theBoltzmann equation to calculate the Tm of theprotein from the fluorescence melt plot.Comparisons can then be made between Tmvalues obtained using a range of bufferconditions, addition of different ligands, or...
Make sure that you first opened the file in the corresponding instrument software first, clicked “Analyze”, and then saved the file, before trying to open with the Protein Thermal Shift™ Software. The file mus...
5. Correlate changes in protein stability or ligand binding to changes in Tm. The Protein Thermal Shift Software calculates the Tm from each fluorescence profile using the Boltzmann method (from a plot of fluorescence intensity vs. temperature) and the derivative method (from a plot of d(...
non-transferable right to perform Life Technologies' patented Protein Thermal Shift™ method may be obtained with purchase of either (a) an Authorized Protein Melt Kit for use in combination with an Applied Biosystems Melt Analysis Software or an Authorized Melt Analysis System or (b) a separate...
(8X), and 5 μl of Protein thermal shift buffer was dispensed in each well. The PCR plates were then sealed with optical seal and centrifuged to remove the air bubbles in the mixture. Thermal scanning (25–99.9 °C at 0.05 °C/s) was done using real-time PCR system (...
31. Hannibal J, Jamen F, Nielsen HS, Journot L, Brabet P, Fahrenkrug J: Dissociation between light-induced phase shift of the circa- dian rhythm and clock gene expression in mice lacking the pituitary adenylate cyclase activating polypeptide type 1 receptor. J Neurosci 2001, 21:4883-4890. ...
was recorded as a function of temperature using PR.ThermControl version 2.3.1 software. Thermal melting curves were plotted using the first derivative of the ratio (350 nm/330 nm). Melting temperatures were calculated automatically by the instrument and represented peaks in the thermal melting...
NMR spectroscopy was chosen to access residue-specific information, and therefore we first obtained chemical shift assignment of the PSD-95 tandem PDZ domain (Supplementary Fig. 9). A comparison of spectra for PSD-95-12 in the unbound form and bound to each clone reveals residue-specific ...