不同的蛋白将具有不同的 Protein Thermal Shift 表达谱,各自具有独特的蛋白熔解曲线形状、斜率、信噪比和温度熔解范围(有关示例,请参见右图)。Protein Thermal Shift 软件通过两种方法从这些曲线生成一个或多个熔解温度值 (Tm):Boltzmann 派生的 Tm 和衍生曲线确定的 Tm,在不同检测条件下,用作曲线间的比较点并...
As a result, we developed the Protein ThermalShiftTM software v1.0 which utilizes theBoltzmann equation to calculate the Tm of theprotein from the fluorescence melt plot.Comparisons can then be made between Tmvalues obtained using a range of bufferconditions, addition of different ligands, or...
Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。节省样品筛查所花费的时间和费用了解更多信息 ...
5. Correlate changes in protein stability or ligand binding to changes in Tm. The Protein Thermal Shift Software calculates the Tm from each fluorescence profile using the Boltzmann method (from a plot of fluorescence intensity vs. temperature) and the derivative method (from a plot of d(...
No right to perform Life Technologies' patented Protein Thermal Shift™ method is conveyed with the purchase of this product. A limited, non-transferable right to perform Life Technologies' patented Protein Thermal Shift™ method may be obtained with purchase of either (a) an Authorized Protein...
(8X), and 5 μl of Protein thermal shift buffer was dispensed in each well. The PCR plates were then sealed with optical seal and centrifuged to remove the air bubbles in the mixture. Thermal scanning (25–99.9 °C at 0.05 °C/s) was done using real-time PCR system (...
was recorded as a function of temperature using PR.ThermControl version 2.3.1 software. Thermal melting curves were plotted using the first derivative of the ratio (350 nm/330 nm). Melting temperatures were calculated automatically by the instrument and represented peaks in the thermal melting...
NMR spectroscopy was chosen to access residue-specific information, and therefore we first obtained chemical shift assignment of the PSD-95 tandem PDZ domain (Supplementary Fig. 9). A comparison of spectra for PSD-95-12 in the unbound form and bound to each clone reveals residue-specific ...
31. Hannibal J, Jamen F, Nielsen HS, Journot L, Brabet P, Fahrenkrug J: Dissociation between light-induced phase shift of the circa- dian rhythm and clock gene expression in mice lacking the pituitary adenylate cyclase activating polypeptide type 1 receptor. J Neurosci 2001, 21:4883-4890. ...
The cellular thermal shift assay (CETSA) was carried out to test the interaction of LRPPRC and GAA in cells32, which showed that GAA could increase the thermal stabilization of LRPPRC (Fig. 2b, c). Immunoblotting showed a decreased LRPPRC protein level in the cells treated by GAA alone or...