Application Note: Protein Thermal Shift Technology Data Sheet: Protein Thermal Shift Dye 有限使用商标许可 ' Notice to Purchaser: The purchase of this Applied Biosystems Melt Analysis Software provides the limited, non-transferable right for the purchaser to perform Life Technologies' patented Protein The...
Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。节省样品筛查所花费的时间和费用了解更多信息 ...
As a result, we developed the Protein ThermalShiftTM software v1.0 which utilizes theBoltzmann equation to calculate the Tm of theprotein from the fluorescence melt plot.Comparisons can then be made between Tmvalues obtained using a range of bufferconditions, addition of different ligands, or...
5. Correlate changes in protein stability or ligand binding to changes in Tm. The Protein Thermal Shift Software calculates the Tm from each fluorescence profile using the Boltzmann method (from a plot of fluorescence intensity vs. temperature) and the derivative method (from a plot of d(...
The melting temperatures (Tm) were taken from the minimum derivative plot (c), plotted as the negative of the first derivative of the normal fluorescent curve, converted by Protein Thermal Shift software. The Tm values were determined as the mean + s.d. of n = 3 replicates. d, e,...
Clear differences in chemical shift positions of MS1–MS3 child A and child B NMR peaks relative to the parent suggest that they adopt distinct folds (Fig. 5e,f; the differences were smaller for MS4 (Supplementary Fig. 24d), which the ACS values (Fig. 5d) and AF2 predictions (...
(8X), and 5 μl of Protein thermal shift buffer was dispensed in each well. The PCR plates were then sealed with optical seal and centrifuged to remove the air bubbles in the mixture. Thermal scanning (25–99.9 °C at 0.05 °C/s) was done using real-time PCR system (...
. The fluorescence intensity was measured during a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s, and protein melting temperatures were calculated based on a Boltzmann function fitting to experimental data, as implemented in the Protein Thermal Shift Software (Applied ...
was recorded as a function of temperature using PR.ThermControl version 2.3.1 software. Thermal melting curves were plotted using the first derivative of the ratio (350 nm/330 nm). Melting temperatures were calculated automatically by the instrument and represented peaks in the thermal melting...
A major challenge in protein design is to augment existing functional proteins with multiple property enhancements. Altering several properties likely necessitates numerous primary sequence changes, and novel methods are needed to accurately predict comb