Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。 节省样品筛查所花费的时间和费用了解更多信息Have...
使用我们的 Protein Thermal Shift™ 试剂盒制备您的样品Protein Thermal Shift 入门试剂盒或 Protein Thermal Shift 染料试剂盒(单独提供)可制备您的分析样品。这些试剂盒允许用户采用与暴露的疏水残基结合的染料,使用实时荧光定量 PCR 仪器监测蛋白的热稳定性,以确定稳定目标蛋白的缓冲条件或筛选结合目标蛋白的化合物...
Biotium公司专利产品众多,包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料Gel Red和GelGreen,以及最新推出的Cheetah Taq热启动酶和CF系列蛋白质标记染料等。 目前,Biotium已将其许多专利技术授权给一些主要的国际生物技术公司,包括安捷伦、BD生物科学、Biolegend、BioRad、EMD Millipore、Illu...
实验方案:Quick Reference Card: Protein Thermal Shift™ Studies: Ligand Screening 技术说明:Protein Thermal Shift染料:热稳定性与光稳定性 技术说明:Protein Thermal Shift技术:优化缓冲液条件和高通量筛选配体-蛋白结合 搜索我们的产品文档数据库:Manuals,Product ...
The Protein Thermal Shift assay can be used to study binding interactions between proteins and antibodies, drugs, and other proteins. When a small-molecule ligand preferentially binds to the native form of a protein, the ligand stabilizes the protein and the Tm of the complex is higher than ...
No right to perform Life Technologies' patented Protein Thermal Shift™ method is conveyed with the purchase of this product. A limited, non-transferable right to perform Life Technologies' patented Protein Thermal Shift™ method may be obtained with purchase of either (a) an Authorized Protein...
The interference of reference and scattered light is detected for each phase shift by a separate camera. Note that the PBS after the objective reflects light out of the image plane and transmits the vertical polarization. PBS, polarizing beamsplitter; NPBS, non-polarizing beamsplitter; QWP/HWP,...
Simulating the mutated proteins compared to the wildtype human Naa10 revealed their impact on its fold, causing a shift in the conformation of two α-helices at the N-terminal harboring most of the interface energy (Supplementary Fig. 5). Thus, mutations can impair the ability to form the ...
Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。节省样品筛查所花费的时间和费用了解更多信息 ...
Applied Biosystems™ Protein Thermal Shift™ 试剂盒使用户可利用与暴露的疏水残基结合的染料,通过实时荧光定量 PCR 仪器来监测蛋白质的热稳定性,从而鉴定使目标蛋白稳定的缓冲液条件或筛选配体库以寻找与目标蛋白结合的化合物。节省样品筛查所花费的时间和费用了解更多信息 ...