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Azenta Life Sciences Sr Business Partnership Mgr, Gene Synthesis Azenta Life Sciences Sr Global Tradeshow & Event Mgr, Tradeshow & Events Azenta Life Sciences Sr Sales Specialist Barts & The London School of Medicine & Dentistry Postdoctoral Research Asst, Antibody Engineering Platform Bayer AG R&D ...
S. cerevisiae remains the key target of yeast-based research and modern tools have elevated our understanding of this host. P. pastoris continues its rise to the forefront of protein production hosts and probably has, together with cell-free protein synthesis (CFPS), the best shot at ...
For studies of BMP7, MAPK14, IL1A, IL1B, TNF, CCL5, IL2, and IFNG expression, mRNA was retrotranscribed with the iScript™ gDNA Clear cDNA Synthesis Kit (BioRad) and analyzed by quantitative PCR using SYBR Green (Life Technologies) with specific primers (Supplementary Table 6) ...
Its earlier variants, including conditional GAN38 and deep convolutional GAN39, were specially developed for a variety of generation tasks including image-to-image translation and text-to-image synthesis. However, these GAN algorithms suffer from problems such as unstable training process, vanishing ...
Single nucleotide alterations: conformational instability and synthesis of aberrant mRNA The potential of genetic alterations to affect protein homeostasis is first highlighted by the numerous base substitution mutations linked to protein conformational diseases, for example in Parkinson’s disease (Chiti and...
MAT2A is responsible for the synthesis of S-adenosylmethionine, which is a common co-substrate involved in methyl group transfer for a variety of different biomolecules. The cellular localization of MAT2A in the nucleus and perinuclear points to a role of SAM production/methylation presumably on ...
Biochemical studies of mammalian oogenesis: protein synthesis during oocyte growth and meiotic maturation in the mouse. J Cell Sci. 1977;24:167–94. CAS PubMed Google Scholar Brinster RL. Protein content of the mouse embryo during the first five days of development. J Reprod Fertil. 1967;13...
RNA extraction, cDNA synthesis, and real-time PCR Cells and zebrafish embryos were harvested for total RNA extraction using TRIzol Reagent (Invitrogen). After DNase I (New England Biolabs) treatment, 2 μg of total RNA for each sample was reverse-transcribed to complementary DNA (cDNA) using...
As far as the driving concentrations for nucleation and aggregation are concerned, in most cases, monomeric concentrations of the amyloid fibril proteins are in the nM to low µM range under physiological conditions, and are tightly regulated by protein synthesis and degradation. Similarly, ...