Protein synthesis refers to the biological process whereby amino acids are assembled by peptide bonding into specific polypeptide sequences in accord with genetic blueprints encoded by deoxyribonucleic acid (DNA). From: Encyclopedia of Stress (Second Edition), 2007 About this pageSet alert Also in sub...
An additional receptor-ligand pair that has gained considerable attention involves CD40 andCD154. CD154, also known asCD40 ligand, is expressed on activated T cells and other cells, including platelets.11,108,121,153CD40 is expressed on APCs. Although the specific effect of CD154 on T cells ...
Once strain construction is complete, the 3D-seq workflow involves simply growing a small volume of the strain under examination, followed by genomic DNA preparation and whole-genome sequencing using the tagmentation library preparation method. Using this protocol, a researcher can theoretically progress...
The presence of 5′-cap on genomic and sub-genomic SARS-CoV-2 mRNAs indicates ability of viral mRNAs to utilize a host translation machinery to synthesize viral proteins. Initiation of translation is a highly regulated step of protein synthesis [6]. It involves sequential assembly 43S, 48S, a...
Here we show that a direct molecular interaction between ICln and the integrin α-chain is not restricted to platelets and involves highly conserved amino acid motifs. Integrin α recruits ICln to the plasma membrane, thereby facilitating the activation of IClswell during hypotonicity. Perturbation ...
The lamins are the major architectural proteins of the animal cell nucleus. Lamins line the inside of the nuclear membrane, where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated
mutation that involves the insertion or deletion of a nucleotide in the DNA sequence Mutagenesis the production of mutations silent mutation A mutation that changes a single nucleotide, but does not change the amino acid created. spontaneous mutations random change in the DNA due to errors in repli...
reactions, mainly focusing on the substrate channel strategy based on DNA and protein scaffold (Schoffelen and Hest2013; Wheeldon2016; Buddingh' BC2017; Nakata et al.2019; Tsitkov2019; Wang et al.2023b). The method by which synthetic multi-enzyme complexes increase product synthesis has long...
We constructed an auxin-inducible degron (AID) system, which utilizes auxin-dependent poly-ubiquitination of Aux/IAA proteins by SCFTIR1 in plants, in fission yeast. Although expression of a plant F-box protein, TIR1, decreased Mcm4-aid, a component of the MCM complex essential for DNA repl...
molecule. The range of sequences that can be probed is only limited by the length of the DNA and the number of unique features that can be effectively synthesized by themicroarraysynthesis technology chosen. In other words, approximately half a million features bearing unique sequences are ...