The tube was then put on a shaker at RT for 2 additional days for labeling. GFP-Booster Alexa Fluor 647 (Chromotek, gb2AF647) and AF568 anti-S1 SARS-CoV-2 (NanoTag, N3501-AF568-L) were used to boost the signal from the GFP and spike protein respectively in the study. Then, the...
Following 1 h incubation on a tabletop orbital shaker, the fluorescent spectra of the SWCNT were recorded using a custom-made nIR microscope array. Briefly, the 96-well plate was placed on top of a motorized stage of a Zeiss AxioVision inverted microscope, and the samples were excited by a ...
Adaptations to near-zero growth rates were dependent on the length of the preceding chemostat phase (Additional file1: Table S1 and Fig. S2). In fact, retentostat cultures that were in chemostat phase for a longer period of time (18 days; 10.8 volume changes (VC)) had significantly lower...
Researchers in academia and industry ideally need to use the same parentalPichiastrain lineage that has already been commercialized because regulatory agencies are familiar with this strain. To achieve this goal, we and others have recently turned to genome sequencing of theK. phaffiitype strains that...
InK. phaffiiand other yeasts, the production of heterologous proteins is generally growth-rate-dependent, typically peaking at intermediate or near maximum specific growth rates (μ) [5,6]. However, the respective relationship between the biomass-specific product formation rate (qP) and μ for a ...
To prepare cytoplasmic extracts, the cells were washed with cold PBS and incubated with a buffer (10 mM Hepes pH 7.9; 1 M KCl; 0.1 mM EDTA; 0.1 mM EGTA, 1:500 protease, and phosphatase inhibitors) for 20 min on ice in a shaker. The cells were then scraped and collect...
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The CHO-K1 mAb cell line was seeded at a density of 2 × 105cells/ml in 50 ml CHO-S-SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170 rpm at 5% CO2. The cultures were grown at 37 °C for 48 h post-seeding, at which ...
TiO2 beads (0.6 mg per 100 µg peptide solution) were added, mixed well, and placed on a shaker for 15 min at room temperature. The solution was then table centrifuged to pellet the beads, which were subsequently mixed with 100 µL loading buffer, transferred to LoBind tube...
Microtiter plates were cultured at 37° C., 300 rpm for 16 hours, shaker orbit is 50 mm. This combination of orbit and minimal shaking speed is required to obtain needed mass transfer coefficient and enable adequate culture oxygenation. After 16 hours of growth, a 1% volume of overnight ...