After click reaction, the mixture was added to a pre-mixed solution (MeOH: CHCl3: H2O = 4 mL: 1 mL: 3 mL) in a 10 mL glass bottle. Samples were mixed and centrifuged at 4500×g for 10 min at room temperature. The bottom and upper layer solution was discarded ...
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The supplement and placebo beverages were placed in opaque shaker bottles with subject numbers placed on the lid of the bottle to ensure anonymity. Athletes were instructed to consume no additional supplements during the study and to refrain from eating for 30 min following the consumption of post...