Thus if there is a three-letter protein alphabet and I attach this alphabet to my sequence, then I would expect that all seq methods can deal with this, e.g. len() or upper() (e.g. by calling seq1). Isn't this one reason for having an alphabet attached to a sequence? Not only...
Nanopores have additional advantages as they detect single-molecules and they are amenable to incorporation into arrays of thousands of nanopores for high throughput analysis. However, despite nanopores having been applied successfully to sequence DNA, their use in protein identification has yet to be ...
The protein-DNA filament scans a duplex molecule for DNA sequence regions complementary to the single strand and can catalyze exchange of the single strand for one strand of the duplex, forming heteroduplex DNA and creating a D-loop (Figure 1B). View chapterExplore book Advances in Cellular ...
We also re-examined the sequence of ISAba13 insertion sites; the strong bias towards AT-rich DNA was lost (Fig. 3d). We conclude that H-NS, rather than underlying DNA sequence, directs ISAba13 to horizontally acquired genes. Fig. 3: Targeting of ISAba13 to AT-rich DNA is driven by...
However, the three-dimensional shape of billions of known protein sequences is not available yet. In this scenario, bioinformatic tools can come to the aid of predicting protein three-dimensional structure with high accuracy, as outlined below [79–85]. Notably, generated models have also ...
Quantitative real-time PCR was performed on an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA) as previously described (8,14). Relative values for mRNA tran- scripts were normalized to the levels of cyclophilin (Ppia). Western Blot Analysis Whole-tissue extracts...
A 10-bit, 3.3-V, 60MSample/s, combined radix<2 and 1.5-bit/stage pipelined analog-to-digital converter A pipeline architecture that combines a new radix<2 gain-stage and the traditional 1.5-bit/stage structures is presented. The 10-bit, 60MHz, 3.3-V pipeline... B Nejati,O Shoaei ...
The invention provides a method for producing type 2 capsid protein grain vaccine of porcine circovirus by a pichia pastoris expression system. The method comprises the following steps of: based on a PCV2-TJ strain gene sequence in GenBank as a template, obtaining a mature coding sequence of ...
Those aptamers with which it interacts are recovered from the library as they have the ability to bind strongly to the target. Since hundreds of modified DNA aptamers are recovered, we utilize standard biotechnology methods to isolate each one and to determine its sequence. The DNA sequence ...
(Fig.S13), presumably due to the exclusive DEGs involved in both studies. Further, GO based functional classification also revealed the exclusiveness in the top enriched GO terms such as “DNA conformation change”, “protein-DNA complex assembly”, “nucleosome organization”, “chromatin assembly...